Human LHCGR ELISA Kit

Alternative name
Human LH/CG-R ELISA Kit;Human Luteinizing hormone receptor ELISA Kit;Human LHR ELISA Kit;Human LSH-R ELISA Kit;Human LCGR ELISA Kit;Human LGR2 ELISA Kit;Human LHRHR ELISA Kit;Human HHG ELISA Kit;Human LH/CGR ELISA Kit;Human ULG5 ELISA Kit;Human luteinizing hormone/choriogonadotropin receptor ELISA Kit;Human lutropin-choriogonadotropic hormone receptor ELISA Kit;Human hypergonadotropic hypogonadism ELISA Kit;Human lutropin/choriogonadotropin receptor ELISA Kit;
Catalog
E023089
species
Human
GeneLHCGR
Standard Curve
Other Species Mouse Lhcgr ELISA Kit Bovine LHCGR ELISA Kit Chicken LHCGR ELISA Kit Porcine LHCGR ELISA Kit Rat Lhcgr ELISA Kit Sheep LHCGR ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of LHCGR. No significant cross-reactivity or interference between LHCGR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LHCGR and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman LHCGR ELISA Kit allows for the in vitro quantitative determination of LHCGR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: LHCGR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LHCGR antibody and an LHCGR-HRP conjugate. The assay sample and buffer are incubated together with LHCGR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LHCGR concentration since LHCGR from samples and LHCGR-HRP conjugate compete for the anti-LHCGR antibody binding site. Since the number of sites is limited, as more sites are occupied by LHCGR from the sample, fewer sites are left to bind LHCGR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LHCGR concentration in each sample is interpolated from this standard curve.
Human Lutropin-choriogonadotropic hormone receptor Protein information
Uniprot ID LSHR_HUMAN
Uniprot AC P22888; Q14751; Q15996; Q9UEW9;
UniGene Hs.468490;
GeneID 3973
KEGG hsa:3973;
Human Lutropin-choriogonadotropic hormone receptor Protein SEQUENCE
SEQUENCE 699 AA; 78643 MW; 2E3D93F4621BA842 CRC64;
MKQRFSALQL LKLLLLLQPP LPRALREALC PEPCNCVPDG ALRCPGPTAG
LTRLSLAYLP VKVIPSQAFR GLNEVIKIEI SQIDSLERIE ANAFDNLLNL
SEILIQNTKN LRYIEPGAFI NLPRLKYLSI CNTGIRKFPD VTKVFSSESN
FILEICDNLH ITTIPGNAFQ GMNNESVTLK LYGNGFEEVQ SHAFNGTTLT
SLELKENVHL EKMHNGAFRG ATGPKTLDIS STKLQALPSY GLESIQRLIA
TSSYSLKKLP SRETFVNLLE ATLTYPSHCC AFRNLPTKEQ NFSHSISENF
SKQCESTVRK VNNKTLYSSM LAESELSGWD YEYGFCLPKT PRCAPEPDAF
NPCEDIMGYD FLRVLIWLIN ILAIMGNMTV LFVLLTSRYK LTVPRFLMCN
LSFADFCMGL YLLLIASVDS QTKGQYYNHA IDWQTGSGCS TAGFFTVFAS
ELSVYTLTVI TLERWHTITY AIHLDQKLRL RHAILIMLGG WLFSSLIAML
PLVGVSNYMK VSICFPMDVE TTLSQVYILT ILILNVVAFF IICACYIKIY
FAVRNPELMA TNKDTKIAKK MAILIFTDFT CMAPISFFAI SAAFKVPLIT
VTNSKVLLVL FYPINSCANP FLYAIFTKTF QRDFFLLLSK FGCCKRRAEL
YRRKDFSAYT SNCKNGFTGS NKPSQSTLKL STLHCQGTAL LDKTRYTEC
UCSC uc002rwu.5; human. [P22888-1];

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Human LHCGR ELISA Kit