Human LRIG3 ELISA Kit

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  • Alternative name

    Human LIG-3 ELISA Kit;Human LIG3 ELISA Kit;Human leucine rich repeats and immunoglobulin like domains 3 ELISA Kit;Human leucine-rich repeats and immunoglobulin-like domains protein 3 ELISA Kit;

  • Catalog
    E023030
  • species
    Human
  • GeneLRIG3
  • Standard CurveHuman LRIG3 ELISA Kit
  • Other Species Mouse Lrig3 ELISA Kit
  • SpecificityNatural and recombinant Human LRIG3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
  • Intended UseHuman LRIG3 ELISA Kit allows for the in vitro quantitative determination of LRIG3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: Human LRIG3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LRIG3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: D28-T807) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LRIG3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LRIG3 amount of sample captured in plate. Background/Introduction: LRIG3 is a 1,119-amino acid protein that contains a signal peptide. This gene is mapped to chromosome 12q13.2. Northern blot analysis detected a 5.1-kb LRIG3 transcript in all tissues analyzed. Quantitative RT-PCR detected highest expression in stomach and lowest expression in blood. And quantitative RT-PCR of mouse tissues revealed ubiquitous expression, but the pattern of expression differed from that in human tissues. LRIG3 may play a role in craniofacial and inner ear morphogenesis during embryonic development. In addition, it may act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.
  • Human Leucine-rich repeats and immunoglobulin-like domains protein 3 Protein information
  • Uniprot ID LRIG3_HUMAN
  • Uniprot AC Q6UXM1; Q6UXL7; Q8NC72;
  • UniGene Hs.253736;
  • GeneID 121227
  • KEGG hsa:121227;
  • Human Leucine-rich repeats and immunoglobulin-like domains protein 3 Protein SEQUENCE
  • SEQUENCE 1119 AA; 123434 MW; EB3B6A391F3C8713 CRC64;

    MSAPSLRARA AGLGLLLCAV LGRAGRSDSG GRGELGQPSG VAAERPCPTT

    CRCLGDLLDC SRKRLARLPE PLPSWVARLD LSHNRLSFIK ASSMSHLQSL

    REVKLNNNEL ETIPNLGPVS ANITLLSLAG NRIVEILPEH LKEFQSLETL

    DLSSNNISEL QTAFPALQLK YLYLNSNRVT SMEPGYFDNL ANTLLVLKLN

    RNRISAIPPK MFKLPQLQHL ELNRNKIKNV DGLTFQGLGA LKSLKMQRNG

    VTKLMDGAFW GLSNMEILQL DHNNLTEITK GWLYGLLMLQ ELHLSQNAIN

    RISPDAWEFC QKLSELDLTF NHLSRLDDSS FLGLSLLNTL HIGNNRVSYI

    ADCAFRGLSS LKTLDLKNNE ISWTIEDMNG AFSGLDKLRR LILQGNRIRS

    ITKKAFTGLD ALEHLDLSDN AIMSLQGNAF SQMKKLQQLH LNTSSLLCDC

    QLKWLPQWVA ENNFQSFVNA SCAHPQLLKG RSIFAVSPDG FVCDDFPKPQ

    ITVQPETQSA IKGSNLSFIC SAASSSDSPM TFAWKKDNEL LHDAEMENYA

    HLRAQGGEVM EYTTILRLRE VEFASEGKYQ CVISNHFGSS YSVKAKLTVN

    MLPSFTKTPM DLTIRAGAMA RLECAAVGHP APQIAWQKDG GTDFPAARER

    RMHVMPEDDV FFIVDVKIED IGVYSCTAQN SAGSISANAT LTVLETPSFL

    RPLLDRTVTK GETAVLQCIA GGSPPPKLNW TKDDSPLVVT ERHFFAAGNQ

    LLIIVDSDVS DAGKYTCEMS NTLGTERGNV RLSVIPTPTC DSPQMTAPSL

    DDDGWATVGV VIIAVVCCVV GTSLVWVVII YHTRRRNEDC SITNTDETNL

    PADIPSYLSS QGTLADRQDG YVSSESGSHH QFVTSSGAGF FLPQHDSSGT

    CHIDNSSEAD VEAATDLFLC PFLGSTGPMY LKGNVYGSDP FETYHTGCSP

    DPRTVLMDHY EPSYIKKKEC YPCSHPSEES CERSFSNISW PSHVRKLLNT

    SYSHNEGPGM KNLCLNKSSL DFSANPEPAS VASSNSFMGT FGKALRRPHL

    DAYSSFGQPS DCQPRAFYLK AHSSPDLDSG SEEDGKERTD FQEENHICTF

    KQTLENYRTP NFQSYDLDT

  • UCSC uc001sqr.5; human. [Q6UXM1-1];



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