Human LCN1 ELISA Kit

Alternative name
Human Tear lipocalin ELISA Kit;Human TLC ELISA Kit;Human tear prealbumin ELISA Kit;Human TP ELISA Kit;Human Von Ebner gland protein ELISA Kit;Human VEG protein ELISA Kit;Human VEGP ELISA Kit;Human PMFA ELISA Kit;Human lipocalin 1 ELISA Kit;Human lipocalin-1 ELISA Kit;Human protein migrating faster than albumin ELISA Kit;
Catalog
E022816
species
Human
GeneLCN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of LCN1. No significant cross-reactivity or interference between LCN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LCN1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman LCN1 ELISA Kit allows for the in vitro quantitative determination of LCN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: LCN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LCN1 antibody and an LCN1-HRP conjugate. The assay sample and buffer are incubated together with LCN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCN1 concentration since LCN1 from samples and LCN1-HRP conjugate compete for the anti-LCN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LCN1 from the sample, fewer sites are left to bind LCN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCN1 concentration in each sample is interpolated from this standard curve.
Human Lipocalin-1 Protein information
Uniprot ID LCN1_HUMAN
Uniprot AC P31025; Q5T8A1;
UniGene Hs.530311;
GeneID 3933
KEGG hsa:3933;
Human Lipocalin-1 Protein SEQUENCE
SEQUENCE 176 AA; 19250 MW; 0DDBF124C8C78CB8 CRC64;
MKPLLLAVSL GLIAALQAHH LLASDEEIQD VSGTWYLKAM TVDREFPEMN
LESVTPMTLT TLEGGNLEAK VTMLISGRCQ EVKAVLEKTD EPGKYTADGG
KHVAYIIRSH VKDHYIFYCE GELHGKPVRG VKLVGRDPKN NLEALEDFEK
AAGARGLSTE SILIPRQSET CSPGSD
UCSC uc004cfz.3; human.;

Human LCN1 ELISA Kit

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