Human MGL ELISA Kit

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  • Alternative name

    Monoglyceride lipase ELISA KIT; HU-K5MGLL ELISA KIT; MAGL ELISA KIT

  • Catalog
    E022793
  • species
    Human
  • GeneMGL
  • Standard CurveHuman MGL ELISA Kit
  • Other Species Mouse MGL ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MGL. No significant cross-reactivity or interference between MGL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MGL is typically less than 0.059ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
  • Intended UseHuman MGL ELISA Kit allows for the in vitro quantitative determination of MGL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MGL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MGL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MGL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MGL in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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