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Human LIMS1 ELISA Kit

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  • Alternative name

    Human particularly interesting new Cys-His protein 1 ELISA Kit;Human PINCH-1 ELISA Kit;Human renal carcinoma antigen NY-REN-48 ELISA Kit;Human PINCH ELISA Kit;Human PINCH1 ELISA Kit;Human LIM zinc finger domain containing 1 ELISA Kit;Human LIM and senescent cell antigen-like-containing domain protein 1 ELISA Kit;Human LIM and senescent cell antigen-like domains 1 ELISA Kit;Human LIM-type zinc finger domains 1 ELISA Kit;Human senescent cell antigen ELISA Kit;
  • Catalog
    E022699
  • species
    Human
  • GeneLIMS1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). No significant cross-reactivity or interference between LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman LIMS1 ELISA Kit allows for the in vitro quantitative determination of LIMS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human LIM and senescent cell antigen-like-containing domain protein 1 Protein information
  • Uniprot ID LIMS1_HUMAN
  • Uniprot AC P48059; B2RAJ4; B7Z483; B7Z7R3; B7Z907; Q53TE0; Q9BS44;
  • UniGene Hs.597715; Hs.613268;
  • GeneID 3987
  • KEGG hsa:3987;
  • Human LIM and senescent cell antigen-like-containing domain protein 1 Protein SEQUENCE

  • SEQUENCE 325 AA; 37251 MW; E665FEB11D849CAE CRC64;

    MANALASATC ERCKGGFAPA EKIVNSNGEL YHEQCFVCAQ CFQQFPEGLF

    YEFEGRKYCE HDFQMLFAPC CHQCGEFIIG RVIKAMNNSW HPECFRCDLC

    QEVLADIGFV KNAGRHLCRP CHNREKARGL GKYICQKCHA IIDEQPLIFK

    NDPYHPDHFN CANCGKELTA DARELKGELY CLPCHDKMGV PICGACRRPI

    EGRVVNAMGK QWHVEHFVCA KCEKPFLGHR HYERKGLAYC ETHYNQLFGD

    VCFHCNRVIE GDVVSALNKA WCVNCFACST CNTKLTLKNK FVEFDMKPVC

    KKCYEKFPLE LKKRLKKLAE TLGRK

  • UCSC uc002teg.4; human. [P48059-1];



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