Human LTA4H ELISA Kit

Alternative name
Human LTA-4 hydrolase ELISA Kit;Human Leukotriene A(4) hydrolase ELISA Kit;Human LTA4 ELISA Kit;Human leukotriene A4 hydrolase ELISA Kit;Human leukotriene A-4 hydrolase ELISA Kit;Human testicular secretory protein Li 27 ELISA Kit;
Catalog
E022646
species
Human
GeneLTA4H
SpecificityThis assay has high sensitivity and excellent specificity for detection of Leukotriene A4 Hydrolase (LTA4H). No significant cross-reactivity or interference between Leukotriene A4 Hydrolase (LTA4H) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.128ng/mL.
Intended UseHuman LTA4H ELISA Kit allows for the in vitro quantitative determination of LTA4H , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukotriene A4 Hydrolase (LTA4H). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukotriene A4 Hydrolase (LTA4H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukotriene A4 Hydrolase (LTA4H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukotriene A4 Hydrolase (LTA4H) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Leukotriene A-4 hydrolase Protein information
Uniprot ID LKHA4_HUMAN
Uniprot AC P09960; B4DNQ9; F8VV40; Q6IAT6; Q9UCT7;
UniGene Hs.524648;
GeneID 4048
KEGG hsa:4048;
Human Leukotriene A-4 hydrolase Protein SEQUENCE
SEQUENCE 611 AA; 69285 MW; 329BF6D04D4A06E1 CRC64;
MPEIVDTCSL ASPASVCRTK HLHLRCSVDF TRRTLTGTAA LTVQSQEDNL
RSLVLDTKDL TIEKVVINGQ EVKYALGERQ SYKGSPMEIS LPIALSKNQE
IVIEISFETS PKSSALQWLT PEQTSGKEHP YLFSQCQAIH CRAILPCQDT
PSVKLTYTAE VSVPKELVAL MSAIRDGETP DPEDPSRKIY KFIQKVPIPC
YLIALVVGAL ESRQIGPRTL VWSEKEQVEK SAYEFSETES MLKIAEDLGG
PYVWGQYDLL VLPPSFPYGG MENPCLTFVT PTLLAGDKSL SNVIAHEISH
SWTGNLVTNK TWDHFWLNEG HTVYLERHIC GRLFGEKFRH FNALGGWGEL
QNSVKTFGET HPFTKLVVDL TDIDPDVAYS SVPYEKGFAL LFYLEQLLGG
PEIFLGFLKA YVEKFSYKSI TTDDWKDFLY SYFKDKVDVL NQVDWNAWLY
SPGLPPIKPN YDMTLTNACI ALSQRWITAK EDDLNSFNAT DLKDLSSHQL
NEFLAQTLQR APLPLGHIKR MQEVYNFNAI NNSEIRFRWL RLCIQSKWED
AIPLALKMAT EQGRMKFTRP LFKDLAAFDK SHDQAVRTYQ EHKASMHPVT
AMLVGKDLKV D
UCSC uc001ten.3; human. [P09960-1];

Category

Human LTA4H ELISA Kit

Human LTA-4 hydrolase ELISA Kit;Human Leukotriene A(4) hydrolase ELISA Kit;Human LTA4 ELISA Kit;Human leukotriene A4 hydrolase ELISA Kit;Human leukotriene A-4 hydrolase ELISA Kit;Human testicular secretory protein Li 27 ELISA Kit;