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Human LIF ELISA Kit

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  • Alternative name

    Human LIF ELISA Kit;Human differentiation-stimulating factor ELISA Kit;Human D factor ELISA Kit;Human melanoma-derived LPL inhibitor ELISA Kit;Human MLPLI ELISA Kit;Human HILDA ELISA Kit;Human CDF ELISA Kit;Human DIA ELISA Kit;Human leukemia inhibitory factor ELISA Kit;Human cholinergic differentiation factor ELISA Kit;Human differentiation inhibitory activity ELISA Kit;Human differentiation-inducing factor ELISA Kit;Human hepatocyte-stimulating factor III ELISA Kit;Human human interleukin in DA cells ELISA Kit;
  • Catalog
    E022552
  • species
    Human
  • GeneLIF
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LIF. No significant cross-reactivity or interference between LIF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LIF and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman LIF ELISA Kit allows for the in vitro quantitative determination of LIF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the assay: LIF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LIF. Standards or samples are then added to the microtiter plate wells and LIF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LIF present in the sample, a standardized prepaHumanion of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LIF are added to each well to "sandwich" the LIF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substHumane solutions are added to each well. The enzyme (HRP) and substHumane are allowed to react over a short incubation period. Only those wells that contain LIF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substHumane reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentHumanion of standards. The LIF concentHumanion in each sample is interpolated from this standard curve.
  • Human Leukemia inhibitory factor Protein information
  • Uniprot ID LIF_HUMAN
  • Uniprot AC P15018; B2RCW7; B5MC23; Q52LZ2;
  • UniGene Hs.2250;
  • GeneID 3976
  • KEGG hsa:3976;
  • Human Leukemia inhibitory factor Protein SEQUENCE

  • SEQUENCE 202 AA; 22008 MW; 634CD10BFF67A217 CRC64;

    MKVLAAGVVP LLLVLHWKHG AGSPLPITPV NATCAIRHPC HNNLMNQIRS

    QLAQLNGSAN ALFILYYTAQ GEPFPNNLDK LCGPNVTDFP PFHANGTEKA

    KLVELYRIVV YLGTSLGNIT RDQKILNPSA LSLHSKLNAT ADILRGLLSN

    VLCRLCSKYH VGHVDVTYGP DTSGKDVFQK KKLGCQLLGK YKQIIAVLAQ

    AF

  • UCSC uc003agz.3; human. [P15018-1];



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