Human HLA-B27 ELISA Kit

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  • Alternative name

    HLA class I histocompatibility antigen, B-27 alpha chain ELISA KIT; MHC class I antigen B*27HLA-B ELISA KIT; HLAB ELISA KIT

  • Catalog
    E022544
  • species
    Human
  • GeneHLA-B27
  • Other Species Mouse HLA-B27 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human HLA-B27. No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.078 ng/ml
  • Intended UseHuman HLA-B27 ELISA Kit allows for the in vitro quantitative determination of HLA-B27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA-B27. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HLA-B27 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HLA-B27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HLA-B27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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