Human LEPR ELISA Kit

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  • Alternative name

    Human LEP-R ELISA Kit;Human huB219 ELISA Kit;Human OB receptor ELISA Kit;Human OB-R ELISA Kit;Human DB ELISA Kit;Human OBR ELISA Kit;Human CD295 ELISA Kit;Human LEPRD ELISA Kit;Human leptin receptor ELISA Kit;

  • Catalog
    E022370
  • species
    Human
  • GeneLEPR
  • Standard CurveHuman LEPR ELISA Kit
  • Other Species Mouse Lepr ELISA KitPorcine LEPR ELISA KitRat Lepr ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Leptin Receptor (LEPR). No significant cross-reactivity or interference between Leptin Receptor (LEPR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 14.3pg/mL.
  • Intended UseHuman LEPR ELISA Kit allows for the in vitro quantitative determination of LEPR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin Receptor (LEPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leptin Receptor (LEPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leptin Receptor (LEPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leptin Receptor (LEPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Leptin receptor Protein information
  • Uniprot ID LEPR_HUMAN
  • Uniprot AC P48357; Q13592; Q13593; Q13594; Q92919; Q92920; Q92921;
  • UniGene Hs.23581; Hs.723178;
  • GeneID 3953
  • KEGG hsa:3953;
  • Human Leptin receptor Protein SEQUENCE
  • SEQUENCE 1165 AA; 132494 MW; CAA03BEAF2602D0A CRC64;

    MICQKFCVVL LHWEFIYVIT AFNLSYPITP WRFKLSCMPP NSTYDYFLLP

    AGLSKNTSNS NGHYETAVEP KFNSSGTHFS NLSKTTFHCC FRSEQDRNCS

    LCADNIEGKT FVSTVNSLVF QQIDANWNIQ CWLKGDLKLF ICYVESLFKN

    LFRNYNYKVH LLYVLPEVLE DSPLVPQKGS FQMVHCNCSV HECCECLVPV

    PTAKLNDTLL MCLKITSGGV IFQSPLMSVQ PINMVKPDPP LGLHMEITDD

    GNLKISWSSP PLVPFPLQYQ VKYSENSTTV IREADKIVSA TSLLVDSILP

    GSSYEVQVRG KRLDGPGIWS DWSTPRVFTT QDVIYFPPKI LTSVGSNVSF

    HCIYKKENKI VPSKEIVWWM NLAEKIPQSQ YDVVSDHVSK VTFFNLNETK

    PRGKFTYDAV YCCNEHECHH RYAELYVIDV NINISCETDG YLTKMTCRWS

    TSTIQSLAES TLQLRYHRSS LYCSDIPSIH PISEPKDCYL QSDGFYECIF

    QPIFLLSGYT MWIRINHSLG SLDSPPTCVL PDSVVKPLPP SSVKAEITIN

    IGLLKISWEK PVFPENNLQF QIRYGLSGKE VQWKMYEVYD AKSKSVSLPV

    PDLCAVYAVQ VRCKRLDGLG YWSNWSNPAY TVVMDIKVPM RGPEFWRIIN

    GDTMKKEKNV TLLWKPLMKN DSLCSVQRYV INHHTSCNGT WSEDVGNHTK

    FTFLWTEQAH TVTVLAINSI GASVANFNLT FSWPMSKVNI VQSLSAYPLN

    SSCVIVSWIL SPSDYKLMYF IIEWKNLNED GEIKWLRISS SVKKYYIHDH

    FIPIEKYQFS LYPIFMEGVG KPKIINSFTQ DDIEKHQSDA GLYVIVPVII

    SSSILLLGTL LISHQRMKKL FWEDVPNPKN CSWAQGLNFQ KPETFEHLFI

    KHTASVTCGP LLLEPETISE DISVDTSWKN KDEMMPTTVV SLLSTTDLEK

    GSVCISDQFN SVNFSEAEGT EVTYEDESQR QPFVKYATLI SNSKPSETGE

    EQGLINSSVT KCFSSKNSPL KDSFSNSSWE IEAQAFFILS DQHPNIISPH

    LTFSEGLDEL LKLEGNFPEE NNDKKSIYYL GVTSIKKRES GVLLTDKSRV

    SCPFPAPCLF TDIRVLQDSC SHFVENNINL GTSSKKTFAS YMPQFQTCST

    QTHKIMENKM CDLTV

  • UCSC uc001dcg.4; human. [P48357-1];



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