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Alternative name
Oxidized low-density lipoprotein receptor 1 ELISA KIT; C-type lectin domain family 8 member A ELISA KIT; Lectin-like oxidized LDL receptor 1 ELISA KIT; LOX-1 ELISA KIT; Lectin-like oxLDL receptor 1 ELISA KIT; hLOX-1OLR1 ELISA KIT; CLEC8A ELISA KIT; LOX1 ELISA KIT; Ox-LDL receptor 1 ELISA KIT; LOX-1 ELISA KIT; Lectin-like oxLDL receptor 1 ELISA KIT; hLOX-1 ELISA KIT
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Catalog
E022285
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species
Human
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GeneLOX1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1).
No significant cross-reactivity or interference between Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.058ng/mL.
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Intended UseHuman LOX1 ELISA Kit allows for the in vitro quantitative determination of LOX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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