Human LRAT ELISA Kit

Alternative name
Human Phosphatidylcholine--retinol O-acyltransferase ELISA Kit;Human LCA14 ELISA Kit;Human lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) ELISA Kit;Human lecithin retinol acyltransferase ELISA Kit;
Catalog
E022277
species
Human
GeneLRAT
SpecificityThis assay has high sensitivity and excellent specificity for detection of human LRAT. No significant cross-reactivity or interference between human LRAT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human LRAT is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman LRAT ELISA Kit allows for the in vitro quantitative determination of LRAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LRAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Lecithin retinol acyltransferase Protein information
Uniprot ID LRAT_HUMAN
Uniprot AC O95237; A8K983; Q8N716;
UniGene Hs.658427; Hs.736130;
GeneID 9227
KEGG hsa:9227;
Human Lecithin retinol acyltransferase Protein SEQUENCE
SEQUENCE 230 AA; 25703 MW; DCB7F5A1C3FF7689 CRC64;
MKNPMLEVVS LLLEKLLLIS NFTLFSSGAA GEDKGRNSFY ETSSFHRGDV
LEVPRTHLTH YGIYLGDNRV AHMMPDILLA LTDDMGRTQK VVSNKRLILG
VIVKVASIRV DTVEDFAYGA NILVNHLDES LQKKALLNEE VARRAEKLLG
FTPYSLLWNN CEHFVTYCRY GTPISPQSDK FCETVKIIIR DQRSVLASAV
LGLASIVCTG LVSYTTLPAI FIPFFLWMAG
UCSC uc003ion.2; human.;

Category

Human LRAT ELISA Kit

Human Phosphatidylcholine--retinol O-acyltransferase ELISA Kit;Human LCA14 ELISA Kit;Human lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) ELISA Kit;Human lecithin retinol acyltransferase ELISA Kit;