Human LCAT ELISA Kit

Alternative name
Human lecithin-cholesterol acyltransferase ELISA Kit;Human phospholipid-cholesterol acyltransferase ELISA Kit;Human phosphatidylcholine-sterol acyltransferase ELISA Kit;Human phosphatidylcholine--sterol O-acyltransferase ELISA Kit;Human testicular secretory protein Li 24 ELISA Kit;
Catalog
E022276
species
Human
GeneLCAT
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lecithin Cholesterol Acyltransferase (LCAT). No significant cross-reactivity or interference between Lecithin Cholesterol Acyltransferase (LCAT) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.18ng/mL.
Intended UseHuman LCAT ELISA Kit allows for the in vitro quantitative determination of LCAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lecithin Cholesterol Acyltransferase (LCAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lecithin Cholesterol Acyltransferase (LCAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Phosphatidylcholine-sterol acyltransferase Protein information
Uniprot ID LCAT_HUMAN
Uniprot AC P04180; Q53XQ3;
UniGene Hs.387239;
GeneID 3931
KEGG hsa:3931;
Human Phosphatidylcholine-sterol acyltransferase Protein SEQUENCE
SEQUENCE 440 AA; 49578 MW; B315EF118AA7A378 CRC64;
MGPPGSPWQW VTLLLGLLLP PAAPFWLLNV LFPPHTTPKA ELSNHTRPVI
LVPGCLGNQL EAKLDKPDVV NWMCYRKTED FFTIWLDLNM FLPLGVDCWI
DNTRVVYNRS SGLVSNAPGV QIRVPGFGKT YSVEYLDSSK LAGYLHTLVQ
NLVNNGYVRD ETVRAAPYDW RLEPGQQEEY YRKLAGLVEE MHAAYGKPVF
LIGHSLGCLH LLYFLLRQPQ AWKDRFIDGF ISLGAPWGGS IKPMLVLASG
DNQGIPIMSS IKLKEEQRIT TTSPWMFPSR MAWPEDHVFI STPSFNYTGR
DFQRFFADLH FEEGWYMWLQ SRDLLAGLPA PGVEVYCLYG VGLPTPRTYI
YDHGFPYTDP VGVLYEDGDD TVATRSTELC GLWQGRQPQP VHLLPLHGIQ
HLNMVFSNLT LEHINAILLG AYRQGPPASP TASPEPPPPE
UCSC uc002euy.2; human.;

Category

Human LCAT ELISA Kit

Human lecithin-cholesterol acyltransferase ELISA Kit;Human phospholipid-cholesterol acyltransferase ELISA Kit;Human phosphatidylcholine-sterol acyltransferase ELISA Kit;Human phosphatidylcholine--sterol O-acyltransferase ELISA Kit;Human testicular secretory protein Li 24 ELISA Kit;