Human LMNB1 ELISA Kit

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  • Alternative name

    Human LMN2 ELISA Kit;Human LMNB ELISA Kit;Human ADLD ELISA Kit;Human LMN ELISA Kit;Human lamin B1 ELISA Kit;Human lamin-B1 ELISA Kit;

  • Catalog
    E022135
  • species
    Human
  • GeneLMNB1
  • Standard CurveHuman LMNB1 ELISA Kit
  • Other Species Mouse Lmnb1 ELISA KitChicken LMNB1 ELISA KitRat Lmnb1 ELISA Kit
  • SpecificityThis assay has high sensitivity for detection of LMNB1. 100% cross-reactivity of LMNB1 was observed among human, mouse and rat.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of LMNB1 is typically less than 0.055ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
  • Intended UseHuman LMNB1 ELISA Kit allows for the in vitro quantitative determination of LMNB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LMNB1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LMNB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LMNB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LMNB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Lamin-B1 Protein information
  • Uniprot ID LMNB1_HUMAN
  • Uniprot AC P20700; B2R6J6; Q3SYN7; Q96EI6;
  • UniGene Hs.89497;
  • GeneID 4001
  • KEGG hsa:4001;
  • Human Lamin-B1 Protein SEQUENCE
  • SEQUENCE 586 AA; 66408 MW; 73292877745722C4 CRC64;

    MATATPVPPR MGSRAGGPTT PLSPTRLSRL QEKEELRELN DRLAVYIDKV

    RSLETENSAL QLQVTEREEV RGRELTGLKA LYETELADAR RALDDTARER

    AKLQIELGKC KAEHDQLLLN YAKKESDLNG AQIKLREYEA ALNSKDAALA

    TALGDKKSLE GDLEDLKDQI AQLEASLAAA KKQLADETLL KVDLENRCQS

    LTEDLEFRKS MYEEEINETR RKHETRLVEV DSGRQIEYEY KLAQALHEMR

    EQHDAQVRLY KEELEQTYHA KLENARLSSE MNTSTVNSAR EELMESRMRI

    ESLSSQLSNL QKESRACLER IQELEDLLAK EKDNSRRMLT DKEREMAEIR

    DQMQQQLNDY EQLLDVKLAL DMEISAYRKL LEGEEERLKL SPSPSSRVTV

    SRASSSRSVR TTRGKRKRVD VEESEASSSV SISHSASATG NVCIEEIDVD

    GKFIRLKNTS EQDQPMGGWE MIRKIGDTSV SYKYTSRYVL KAGQTVTIWA

    ANAGVTASPP TDLIWKNQNS WGTGEDVKVI LKNSQGEEVA QRSTVFKTTI

    PEEEEEEEEA AGVVVEEELF HQQGTPRASN RSCAIM

  • UCSC uc003kud.3; human.;


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