Human LF ELISA Kit

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  • Alternative name

    Lactotransferrin ELISA KIT; Growth-inhibiting protein 12 ELISA KIT; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H ELISA KIT; Lfcin-H ELISA KIT; Kaliocin-1 ELISA KIT; Lactoferroxin-A ELISA KIT; Lactoferroxin-B ELISA KIT; Lactoferroxin-CLTF ELISA KIT; GIG12 ELISA KIT; LF ELISA KIT; Lactoferrin ELISA KIT; Lfcin-H ELISA KIT

  • Catalog
    E022093
  • species
    Human
  • GeneLF
  • Standard CurveHuman LF ELISA Kit
  • Other Species Human LTF/LF ELISA KitHuman LF / LTF Ab-IgG ELISA KitHuman LF/LTF-Ab-IgG ELISA KitMouse LF ELISA KitMouse Anti-LF ELISA KitMouse LF/LTF Ab-IgG ELISA KitMouse LF / LTF Ab-IgG ELISA KitMouse LTF/LF ELISA KitMouse LF/LTF ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman LF ELISA Kit allows for the in vitro quantitative determination of LF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Intended Uses: This LTF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTF. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: LTF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LTF. Standards or samples are then added to the microtiter plate wells and LTF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LTF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LTF are added to each well to "sandwich" the LTF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LTF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTF concentration in each sample is interpolated from this standard curve.



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