Human LDHD ELISA Kit

Alternative name
Human DLD ELISA Kit;Human lactate dehydrogenase D ELISA Kit;Human probable D-lactate dehydrogenase, mitochondrial ELISA Kit;Human D-lactate dehydrogenase ELISA Kit;
Catalog
E022081
species
Human
GeneLDHD
Other Species Mouse Ldhd ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human LDHD. No significant cross-reactivity or interference between Human LDHD and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7.8 mIU/ml
Intended UseHuman LDHD ELISA Kit allows for the in vitro quantitative determination of LDHD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. LDH is found in the liver, kidneys, striated muscle, and heart muscle that catalyze the reversible conversion of pyruvate and lactate. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It converts pyruvate, the final product of glycolysis to lactic acid when oxygen is absent or in short supply, and it performs the reverse reaction during the cori cycle in the liver. At high concentrations of lactate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. Tissue breakdown elevates levels of LDH, and therefore a measure of it indicates e.g. hemolysis. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis and HIV. Two forms of lactate dehydrogenase with different substrate specificities have been found; L-lactate dehydrogenase and D-lactate dehydrogenase. Whereas the enzyme from vertebrates and plants only forms L-lactate, invertebrates, lower fungi, and prokaryotic organisms possess either a D- or L-lactate specific dehydrogenase, depending on the species. Some prokaryotes even possess both enzymes within the same cell. On the other hand, eukaryotes have several isozyme forms, but all have a single stereospecificity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well 4 and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Probable D-lactate dehydrogenase, mitochondrial Protein information
Uniprot ID LDHD_HUMAN
Uniprot AC Q86WU2; Q8IZK5;
UniGene Hs.380929;
GeneID 197257
KEGG hsa:197257;
Human Probable D-lactate dehydrogenase, mitochondrial Protein SEQUENCE
SEQUENCE 507 AA; 54871 MW; 5625743709939182 CRC64;
MARLLRSATW ELFPWRGYCS QKAKGELCRD FVEALKAVVG GSHVSTAAVV
REQHGRDESV HRCEPPDAVV WPQNVEQVSR LAALCYRQGV PIIPFGTGTG
LEGGVCAVQG GVCVNLTHMD RILELNQEDF SVVVEPGVTR KALNAHLRDS
GLWFPVDPGA DASLCGMAAT GASGTNAVRY GTMRDNVLNL EVVLPDGRLL
HTAGRGRHFR FGFWPEIPHH TAWYSPCVSL GRRKSAAGYN LTGLFVGSEG
TLGLITATTL RLHPAPEATV AATCAFPSVQ AAVDSTVHIL QAAVPVARIE
FLDEVMMDAC NRYSKLNCLV APTLFLEFHG SQQALEEQLQ RTEEIVQQNG
ASDFSWAKEA EERSRLWTAR HNAWYAALAT RPGCKGYSTD VCVPISRLPE
IVVQTKEDLN ASGLTGSIVG HVGDGNFHCI LLVNPDDAEE LGRVKAFAEQ
LGRRALALHG TCTGEHGIGM GKRQLLQEEV GAVGVETMRQ LKAVLDPQGL
MNPGKVL
UCSC uc002fdm.4; human. [Q86WU2-1];

Related products with Human LDHD ELISA Kit

anti-LDHD antibody

Category

Human LDHD ELISA Kit

Human DLD ELISA Kit;Human lactate dehydrogenase D ELISA Kit;Human probable D-lactate dehydrogenase, mitochondrial ELISA Kit;Human D-lactate dehydrogenase ELISA Kit;