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Human LDHA ELISA Kit

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  • Alternative name

    Human LDH-A ELISA Kit;Human cell proliferation-inducing gene 19 protein ELISA Kit;Human LDH muscle subunit ELISA Kit;Human LDH-M ELISA Kit;Human renal carcinoma antigen NY-REN-59 ELISA Kit;Human GSD11 ELISA Kit;Human HEL-S-133P ELISA Kit;Human LDHM ELISA Kit;Human PIG19 ELISA Kit;Human lactate dehydrogenase A ELISA Kit;Human L-lactate dehydrogenase A chain ELISA Kit;Human epididymis secretory sperm binding protein Li 133P ELISA Kit;Human lactate dehydrogenase M ELISA Kit;Human proliferation-inducing gene 19 ELISA Kit;
  • Catalog
    E022070
  • species
    Human
  • GeneLDHA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Lactate Dehydrogenase A (LDHA). No significant cross-reactivity or interference between Lactate Dehydrogenase A (LDHA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseHuman LDHA ELISA Kit allows for the in vitro quantitative determination of LDHA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase A (LDHA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase A (LDHA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase A (LDHA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactate Dehydrogenase A (LDHA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human L-lactate dehydrogenase A chain Protein information
  • Uniprot ID LDHA_HUMAN
  • Uniprot AC Q9UDE8; Q9UDE9;
  • UniGene Hs.2795;
  • GeneID 3939
  • KEGG hsa:3939;
  • Human L-lactate dehydrogenase A chain Protein SEQUENCE

  • SEQUENCE 332 AA; 36689 MW; 401E8604CEB7F908 CRC64;

    MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL

    VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ

    QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI

    SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV

    WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW

    AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI

    SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF

  • UCSC uc001mol.4; human. [P00338-1];



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