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Human L1CAM ELISA Kit

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  • Alternative name

    Human N-CAM-L1 ELISA Kit;Human NCAM-L1 ELISA Kit;Human CAML1 ELISA Kit;Human MIC5 ELISA Kit;Human CD171 ELISA Kit;Human HSAS ELISA Kit;Human HSAS1 ELISA Kit;Human MASA ELISA Kit;Human N-CAML1 ELISA Kit;Human S10 ELISA Kit;Human SPG1 ELISA Kit;Human L1 cell adhesion molecule ELISA Kit;Human neural cell adhesion molecule L1 ELISA Kit;Human antigen identified by monoclonal antibody R1 ELISA Kit;
  • Catalog
    E022038
  • species
    Human
  • GeneL1CAM
  • Standard CurveHuman L1CAM ELISA Kit
  • Other Species Human L1CAM/CD171 ELISA KitMouse L1cam ELISA KitRat L1cam ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of L1CAM. No significant cross-reactivity or interference between L1CAM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between L1CAM and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman L1CAM ELISA Kit allows for the in vitro quantitative determination of L1CAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the assay: L1CAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-L1CAM antibody and an L1CAM-HRP conjugate. The assay sample and buffer are incubated together with L1CAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the L1CAM concentration since L1CAM from samples and L1CAM-HRP conjugate compete for the anti-L1CAM antibody binding site. Since the number of sites is limited, as more sites are occupied by L1CAM from the sample, fewer sites are left to bind L1CAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L1CAM concentration in each sample is interpolated from this standard curve.
  • Human Neural cell adhesion molecule L1 Protein information
  • Uniprot ID L1CAM_HUMAN
  • Uniprot AC P32004; A0AV65; A4ZYW4; B2RMU7; G3XAF4; Q8TA87;
  • UniGene Hs.522818;
  • GeneID 3897
  • KEGG hsa:3897;
  • Human Neural cell adhesion molecule L1 Protein SEQUENCE

  • SEQUENCE 1257 AA; 140003 MW; 5EDD764DA86C0E63 CRC64;

    MVVALRYVWP LLLCSPCLLI QIPEEYEGHH VMEPPVITEQ SPRRLVVFPT

    DDISLKCEAS GKPEVQFRWT RDGVHFKPKE ELGVTVYQSP HSGSFTITGN

    NSNFAQRFQG IYRCFASNKL GTAMSHEIRL MAEGAPKWPK ETVKPVEVEE

    GESVVLPCNP PPSAEPLRIY WMNSKILHIK QDERVTMGQN GNLYFANVLT

    SDNHSDYICH AHFPGTRTII QKEPIDLRVK ATNSMIDRKP RLLFPTNSSS

    HLVALQGQPL VLECIAEGFP TPTIKWLRPS GPMPADRVTY QNHNKTLQLL

    KVGEEDDGEY RCLAENSLGS ARHAYYVTVE AAPYWLHKPQ SHLYGPGETA

    RLDCQVQGRP QPEVTWRING IPVEELAKDQ KYRIQRGALI LSNVQPSDTM

    VTQCEARNRH GLLLANAYIY VVQLPAKILT ADNQTYMAVQ GSTAYLLCKA

    FGAPVPSVQW LDEDGTTVLQ DERFFPYANG TLGIRDLQAN DTGRYFCLAA

    NDQNNVTIMA NLKVKDATQI TQGPRSTIEK KGSRVTFTCQ ASFDPSLQPS

    ITWRGDGRDL QELGDSDKYF IEDGRLVIHS LDYSDQGNYS CVASTELDVV

    ESRAQLLVVG SPGPVPRLVL SDLHLLTQSQ VRVSWSPAED HNAPIEKYDI

    EFEDKEMAPE KWYSLGKVPG NQTSTTLKLS PYVHYTFRVT AINKYGPGEP

    SPVSETVVTP EAAPEKNPVD VKGEGNETTN MVITWKPLRW MDWNAPQVQY

    RVQWRPQGTR GPWQEQIVSD PFLVVSNTST FVPYEIKVQA VNSQGKGPEP

    QVTIGYSGED YPQAIPELEG IEILNSSAVL VKWRPVDLAQ VKGHLRGYNV

    TYWREGSQRK HSKRHIHKDH VVVPANTTSV ILSGLRPYSS YHLEVQAFNG

    RGSGPASEFT FSTPEGVPGH PEALHLECQS NTSLLLRWQP PLSHNGVLTG

    YVLSYHPLDE GGKGQLSFNL RDPELRTHNL TDLSPHLRYR FQLQATTKEG

    PGEAIVREGG TMALSGISDF GNISATAGEN YSVVSWVPKE GQCNFRFHIL

    FKALGEEKGG ASLSPQYVSY NQSSYTQWDL QPDTDYEIHL FKERMFRHQM

    AVKTNGTGRV RLPPAGFATE GWFIGFVSAI ILLLLVLLIL CFIKRSKGGK

    YSVKDKEDTQ VDSEARPMKD ETFGEYRSLE SDNEEKAFGS SQPSLNGDIK

    PLGSDDSLAD YGGSVDVQFN EDGSFIGQYS GKKEKEAAGG NDSSGATSPI

    NPAVALE

  • UCSC uc004fjc.5; human. [P32004-1];


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