Human KLF17 ELISA Kit

Alternative name
Human zinc finger protein 393 ELISA Kit;Human ZNF393 ELISA Kit;Human Zfp393 ELISA Kit;Human Kruppel like factor 17 ELISA Kit;Human Krueppel-like factor 17 ELISA Kit;Human novel zinc-finger protein ELISA Kit;
Catalog
E021954
species
Human
GeneKLF17
Standard Curve
Other Species Mouse Klf17 ELISA Kit Porcine KLF17 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of KLF17. No significant cross-reactivity or interference between KLF17 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KLF17 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman KLF17 ELISA Kit allows for the in vitro quantitative determination of KLF17 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: KLF17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLF17 antibody and an KLF17-HRP conjugate. The assay sample and buffer are incubated together with KLF17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLF17 concentration since KLF17 from samples and KLF17-HRP conjugate compete for the anti-KLF17 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLF17 from the sample, fewer sites are left to bind KLF17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF17 concentration in each sample is interpolated from this standard curve.
Human Krueppel-like factor 17 Protein information
Uniprot ID KLF17_HUMAN
Uniprot AC Q5JT82; Q86VQ7; Q8N805;
UniGene Hs.567674; Hs.647667;
GeneID 128209
KEGG hsa:128209;
Human Krueppel-like factor 17 Protein SEQUENCE
SEQUENCE 389 AA; 42577 MW; 0AA451DB23C2580A CRC64;
MYGRPQAEME QEAGELSRWQ AAHQAAQDNE NSAPILNMSS SSGSSGVHTS
WNQGLPSIQH FPHSAEMLGS PLVSVEAPGQ NVNEGGPQFS MPLPERGMSY
CPQATLTPSR MIYCQRMSPP QQEMTIFSGP QLMPVGEPNI PRVARPFGGN
LRMPPNGLPV SASTGIPIMS HTGNPPVPYP GLSTVPSDET LLGPTVPSTE
AQAVLPSMAQ MLPPQDAHDL GMPPAESQSL LVLGSQDSLV SQPDSQEGPF
LPEQPGPAPQ TVEKNSRPQE GTGRRGSSEA RPYCCNYENC GKAYTKRSHL
VSHQRKHTGE RPYSCNWESC SWSFFRSDEL RRHMRVHTRY RPYKCDQCSR
EFMRSDHLKQ HQKTHRPGPS DPQANNNNGE QDSPPAAGP
UCSC uc001clp.4; human.;

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anti-KLF17 antibody

Category

Human KLF17 ELISA Kit

Human zinc finger protein 393 ELISA Kit;Human ZNF393 ELISA Kit;Human Zfp393 ELISA Kit;Human Kruppel like factor 17 ELISA Kit;Human Krueppel-like factor 17 ELISA Kit;Human novel zinc-finger protein ELISA Kit;