Human KIF18A ELISA Kit

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  • Alternative name

    Human Marrow stromal KIF18A ELISA Kit;Human MS-KIF18A ELISA Kit;Human PPP1R99 ELISA Kit;Human kinesin family member 18A ELISA Kit;Human kinesin-like protein KIF18A ELISA Kit;Human protein phosphatase 1, regulatory subunit 99 ELISA Kit;

  • Catalog
    E021857
  • species
    Human
  • GeneKIF18A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Kinesin Family, Member 18A (KIF18A). No significant cross-reactivity or interference between Kinesin Family, Member 18A (KIF18A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.127ng/mL.
  • Intended UseHuman KIF18A ELISA Kit allows for the in vitro quantitative determination of KIF18A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 18A (KIF18A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Family, Member 18A (KIF18A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 18A (KIF18A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin Family, Member 18A (KIF18A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Kinesin-like protein KIF18A Protein information
  • Uniprot ID KI18A_HUMAN
  • Uniprot AC Q8NI77; Q4VPE3; Q86VS5; Q9H0F3;
  • UniGene Hs.301052;
  • GeneID 81930
  • KEGG hsa:81930;
  • Human Kinesin-like protein KIF18A Protein SEQUENCE
  • SEQUENCE 898 AA; 102281 MW; 6DFD904378BF9B27 CRC64;

    MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE

    VSFFHGKKTT NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS

    FLNGYNCTVL AYGATGAGKT HTMLGSADEP GVMYLTMLHL YKCMDEIKEE

    KICSTAVSYL EVYNEQIRDL LVNSGPLAVR EDTQKGVVVH GLTLHQPKSS

    EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD KTASINQNVR

    IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR

    KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN

    RAKDIKSSLK SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF

    TNENDQAKLM ISNPQEKEIE RFQEILNCLF QNREEIRQEY LKLEMLLKEN

    ELKSFYQQQC HKQIEMMCSE DKVEKATGKR DHRLAMLKTR RSYLEKRREE

    ELKQFDENTN WLHRVEKEMG LLSQNGHIPK ELKKDLHCHH LHLQNKDLKA

    QIRHMMDLAC LQEQQHRQTE AVLNALLPTL RKQYCTLKEA GLSNAAFESD

    FKEIEHLVER KKVVVWADQT AEQPKQNDLP GISVLMTFPQ LGPVQPIPCC

    SSSGGTNLVK IPTEKRTRRK LMPSPLKGQH TLKSPPSQSV QLNDSLSKEL

    QPIVYTPEDC RKAFQNPSTV TLMKPSSFTT SFQAISSNIN SDNCLKMLCE

    VAIPHNRRKE CGQEDLDSTF TICEDIKSSK CKLPEQESLP NDNKDILQRL

    DPSSFSTKHS MPVPSMVPSY MAMTTAAKRK RKLTSSTSNS SLTADVNSGF

    AKRVRQDNSS EKHLQENKPT MEHKRNICKI NPSMVRKFGR NISKGNLR

  • UCSC uc001msc.3; human.;



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