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Catalog
E002182
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species
Human
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GeneAngIII
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Standard Curve
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiotensin III (AngIII).
No significant cross-reactivity or interference between Angiotensin III (AngIII) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 2.77pg/mL.
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Intended UseHuman AngIII ELISA Kit allows for the in vitro quantitative determination of AngIII , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin III (AngIII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin III (AngIII) and unlabeled Angiotensin III (AngIII) (Standards or samples) with the pre-coated antibody specific to Angiotensin III (AngIII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin III (AngIII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin III (AngIII) in the sample.
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