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Alternative name
Vascular endothelial growth factor receptor 2 ELISA KIT; Fetal liver kinase 1 ELISA KIT; FLK-1 ELISA KIT; Kinase insert domain receptor ELISA KIT; KDR ELISA KIT; Protein-tyrosine kinase receptor flk-1 ELISA KIT; CD_antigen: CD309KDR ELISA KIT; FLK1 ELISA KIT; VEGFR2 ELISA KIT; VEGFR-2 ELISA KIT; FLK-1 ELISA KIT; KDR ELISA KIT
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Catalog
E021806
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species
Human
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GeneVEGFR2
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human KDR. No significant cross-reactivity or interference between Human KDR and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.144ng/ml
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Intended UseHuman VEGFR2 ELISA Kit allows for the in vitro quantitative determination of VEGFR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGFR-2/Flk-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGFR-2/Flk-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFR-2/Flk-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFR-2/Flk-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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