Human AGTR2 ELISA Kit

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  • Alternative name

    Human angiotensin II type-2 receptor ELISA Kit;Human AT2 ELISA Kit;Human ATGR2 ELISA Kit;Human MRX88 ELISA Kit;Human angiotensin II receptor type 2 ELISA Kit;Human type-2 angiotensin II receptor ELISA Kit;

  • Catalog
    E002167
  • species
    Human
  • GeneAGTR2
  • Standard CurveHuman AGTR2 ELISA Kit
  • Other Species Human AGTR2-AA ELISA KitHuman AGTR2 AA ELISA KitMouse AGTR2-AA ELISA KitMouse Agtr2 ELISA KitRat Agtr2 ELISA KitSheep AGTR2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman AGTR2 ELISA Kit allows for the in vitro quantitative determination of AGTR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: AGTR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGTR2 antibody and an AGTR2-HRP conjugate. The assay sample and buffer are incubated together with AGTR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGTR2 concentration since AGTR2 from samples and AGTR2-HRP conjugate compete for the anti-AGTR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGTR2 from the sample, fewer sites are left to bind AGTR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGTR2 concentration in each sample is interpolated from this standard curve.
  • Human Type-2 angiotensin II receptor Protein information
  • Uniprot ID AGTR2_HUMAN
  • Uniprot AC P50052; B2R9V1; Q13016; Q6FGY7;
  • UniGene Hs.405348;
  • GeneID 186
  • KEGG hsa:186;
  • Human Type-2 angiotensin II receptor Protein SEQUENCE
  • SEQUENCE 363 AA; 41184 MW; FDD7D4E6F9B43E60 CRC64;

    MKGNSTLATT SKNITSGLHF GLVNISGNNE STLNCSQKPS DKHLDAIPIL

    YYIIFVIGFL VNIVVVTLFC CQKGPKKVSS IYIFNLAVAD LLLLATLPLW

    ATYYSYRYDW LFGPVMCKVF GSFLTLNMFA SIFFITCMSV DRYQSVIYPF

    LSQRRNPWQA SYIVPLVWCM ACLSSLPTFY FRDVRTIEYL GVNACIMAFP

    PEKYAQWSAG IALMKNILGF IIPLIFIATC YFGIRKHLLK TNSYGKNRIT

    RDQVLKMAAA VVLAFIICWL PFHVLTFLDA LAWMGVINSC EVIAVIDLAL

    PFAILLGFTN SCVNPFLYCF VGNRFQQKLR SVFRVPITWL QGKRESMSCR

    KSSSLREMET FVS

  • UCSC uc004eqh.5; human.;


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