Human KHK ELISA Kit

Alternative name
Human hepatic fructokinase ELISA Kit;Human ketohexokinase ELISA Kit;Human fructokinase ELISA Kit;Human testicular tissue protein Li 102 ELISA Kit;
Catalog
E021627
species
Human
GeneKHK
SpecificityThis assay has high sensitivity and excellent specificity for detection of human KHK. No significant cross-reactivity or interference between human KHK and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human KHK is typically less than 4.68 mU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman KHK ELISA Kit allows for the in vitro quantitative determination of KHK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KHK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KHK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KHK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KHK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Ketohexokinase Protein information
Uniprot ID KHK_HUMAN
Uniprot AC P50053; Q6IBK2; Q99532; Q9BRJ3; Q9UMN1;
UniGene Hs.567297;
GeneID 3795
KEGG hsa:3795;
Human Ketohexokinase Protein SEQUENCE
SEQUENCE 298 AA; 32523 MW; BE77FC325CAC5721 CRC64;
MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTVL
SLLGAPCAFM GSMAPGHVAD FLVADFRRRG VDVSQVAWQS KGDTPSSCCI
INNSNGNRTI VLHDTSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML
QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG
FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV
VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIV
UCSC uc002ril.3; human. [P50053-1];

Category

Human KHK ELISA Kit

Human hepatic fructokinase ELISA Kit;Human ketohexokinase ELISA Kit;Human fructokinase ELISA Kit;Human testicular tissue protein Li 102 ELISA Kit;