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Human KRT77 ELISA Kit

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  • Alternative name

    Human cytokeratin-1B ELISA Kit;Human CK-1B ELISA Kit;Human Keratin-77 ELISA Kit;Human K77 ELISA Kit;Human type-II keratin Kb39 ELISA Kit;Human KRT1B ELISA Kit;Human K1B ELISA Kit;Human keratin 77 ELISA Kit;Human keratin, type II cytoskeletal 1b ELISA Kit;Human keratin 1B ELISA Kit;Human keratin 77, type II ELISA Kit;
  • Catalog
    E021495
  • species
    Human
  • GeneKRT77
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human KRT77. No significant cross-reactivity or interference between human KRT77 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human KRT77 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman KRT77 ELISA Kit allows for the in vitro quantitative determination of KRT77 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRT77 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT77 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT77 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT77 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Keratin, type II cytoskeletal 1b Protein information
  • Uniprot ID K2C1B_HUMAN
  • Uniprot AC Q7Z794; Q7RTS8;
  • UniGene Hs.334989;
  • GeneID 374454
  • KEGG hsa:374454;
  • Human Keratin, type II cytoskeletal 1b Protein SEQUENCE

  • SEQUENCE 578 AA; 61901 MW; 232F92351DF0FDCA CRC64;

    MSHQFSSQSA FSSMSRRVYS TSSSAGSGGG SPAVGSVCYA RGRCGGGGYG

    IHGRGFGSRS LYNLGGSRSI SINLMGRSTS GFCQGGGVGG FGGGRGFGVG

    STGAGGFGGG GFGGAGFGTS NFGLGGFGPY CPPGGIQEVT INQSLLEPLH

    LEVDPEIQRI KTQEREQIMV LNNKFASFID KVRFLEQQNQ VLQTKWELLQ

    QVNTSTGTNN LEPLLENYIG DLRRQVDLLS AEQMRQNAEV RSMQDVVEDY

    KSKYEDEINK RTGSENDFVV LKKDVDAAYV SKVDLESRVD TLTGEVNFLK

    YLFLTELSQV QTHISDTNVI LSMDNNRSLD LDSIIDAVRT QYELIAQRSK

    DEAEALYQTK YQELQITAGR HGDDLKNSKM EIAELNRTVQ RLQAEISNVK

    KQIEQMQSLI SDAEERGEQA LQDAWQKLQD LEEALQQSKE ELARLLRDYQ

    AMLGVKLSLD VEIATYRQLL EGEESRMSGE LQSHVSISVQ NSQVSVNGGA

    GGGGSYGSGG YGGGSGGGYG GGRSYRGGGA RGRSGGGYGS GCGGGGGSYG

    GSGRSGRGSS RVQIIQTSTN TSHRRILE

  • UCSC uc001saw.4; human.;



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