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Human KRT5 ELISA Kit

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  • Alternative name

    Human 58 kda cytokeratin ELISA Kit;Human cytokeratin-5 ELISA Kit;Human CK-5 ELISA Kit;Human Keratin-5 ELISA Kit;Human K5 ELISA Kit;Human type-II keratin Kb5 ELISA Kit;Human CK5 ELISA Kit;Human DDD ELISA Kit;Human DDD1 ELISA Kit;Human EBS2 ELISA Kit;Human KRT5A ELISA Kit;Human keratin 5 ELISA Kit;Human keratin, type II cytoskeletal 5 ELISA Kit;Human epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types ELISA Kit;Human keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) ELISA Kit;Human keratin 5, type II ELISA Kit;
  • Catalog
    E021480
  • species
    Human
  • GeneKRT5
  • Standard CurveHuman KRT5 ELISA Kit
  • Other Species Mouse Krt5 ELISA KitBovine KRT5 ELISA KitRat Krt5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 5 (KRT5). No significant cross-reactivity or interference between Keratin 5 (KRT5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.5pg/mL.
  • Intended UseHuman KRT5 ELISA Kit allows for the in vitro quantitative determination of KRT5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 5 (KRT5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 5 (KRT5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 5 (KRT5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 5 (KRT5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Keratin, type II cytoskeletal 5 Protein information
  • Uniprot ID K2C5_HUMAN
  • Uniprot AC P13647; Q6PI71; Q6UBJ0; Q8TA91;
  • UniGene Hs.433845;
  • GeneID 3852
  • KEGG hsa:3852;
  • Human Keratin, type II cytoskeletal 5 Protein SEQUENCE

  • SEQUENCE 590 AA; 62378 MW; E9D5318E01F55145 CRC64;

    MSRQSSVSFR SGGSRSFSTA SAITPSVSRT SFTSVSRSGG GGGGGFGRVS

    LAGACGVGGY GSRSLYNLGG SKRISISTSG GSFRNRFGAG AGGGYGFGGG

    AGSGFGFGGG AGGGFGLGGG AGFGGGFGGP GFPVCPPGGI QEVTVNQSLL

    TPLNLQIDPS IQRVRTEERE QIKTLNNKFA SFIDKVRFLE QQNKVLDTKW

    TLLQEQGTKT VRQNLEPLFE QYINNLRRQL DSIVGERGRL DSELRNMQDL

    VEDFKNKYED EINKRTTAEN EFVMLKKDVD AAYMNKVELE AKVDALMDEI

    NFMKMFFDAE LSQMQTHVSD TSVVLSMDNN RNLDLDSIIA EVKAQYEEIA

    NRSRTEAESW YQTKYEELQQ TAGRHGDDLR NTKHEISEMN RMIQRLRAEI

    DNVKKQCANL QNAIADAEQR GELALKDARN KLAELEEALQ KAKQDMARLL

    REYQELMNTK LALDVEIATY RKLLEGEECR LSGEGVGPVN ISVVTSSVSS

    GYGSGSGYGG GLGGGLGGGL GGGLAGGSSG SYYSSSSGGV GLGGGLSVGG

    SGFSASSGRG LGVGFGSGGG SSSSVKFVST TSSSRKSFKS

  • UCSC uc001san.4; human.;



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