Human KRT4 ELISA Kit

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  • Alternative name

    Human Cytokeratin-4 ELISA Kit;Human CK-4 ELISA Kit;Human Keratin-4 ELISA Kit;Human K4 ELISA Kit;Human Type-II keratin Kb4 ELISA Kit;Human CYK4 ELISA Kit;

  • Catalog
    E021475
  • species
    Human
  • GeneKRT4
  • Standard CurveHuman KRT4 ELISA Kit
  • Other Species Mouse Krt4 ELISA KitRat Krt4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 4 (KRT4). No significant cross-reactivity or interference between Keratin 4 (KRT4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman KRT4 ELISA Kit allows for the in vitro quantitative determination of KRT4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 4 (KRT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 4 (KRT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 4 (KRT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 4 (KRT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Keratin, type II cytoskeletal 4 Protein information
  • Uniprot ID K2C4_HUMAN
  • Uniprot AC P19013; F8VS64; Q6GTR8; Q96LA7; Q9BTL1;
  • UniGene Hs.654610; Hs.731814;
  • Human Keratin, type II cytoskeletal 4 Protein SEQUENCE
  • SEQUENCE 534 AA; 57285 MW; 3795B7C93092F97E CRC64;

    MIARQQCVRG GPRGFSCGSA IVGGGKRGAF SSVSMSGGAG RCSSGGFGSR

    SLYNLRGNKS ISMSVAGSRQ GACFGGAGGF GTGGFGAGGF GAGFGTGGFG

    GGFGGSFSGK GGPGFPVCPA GGIQEVTINQ SLLTPLHVEI DPEIQKVRTE

    EREQIKLLNN KFASFIDKVQ FLEQQNKVLE TKWNLLQQQT TTTSSKNLEP

    LFETYLSVLR KQLDTLGNDK GRLQSELKTM QDSVEDFKTK YEEEINKRTA

    AENDFVVLKK DVDAAYLNKV ELEAKVDSLN DEINFLKVLY DAELSQMQTH

    VSDTSVVLSM DNNRNLDLDS IIAEVRAQYE EIAQRSKAEA EALYQTKVQQ

    LQISVDQHGD NLKNTKSEIA ELNRMIQRLR AEIENIKKQC QTLQVSVADA

    EQRGENALKD AHSKRVELEA ALQQAKEELA RMLREYQELM SVKLALDIEI

    ATYRKLLEGE EYRMSGECQS AVSISVVSGS TSTGGISGGL GSGSGFGLSS

    GFGSGSGSGF GFGGSVSGSS SSKIISTTTL NKRR

  • UCSC uc031qhk.2; human.;


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