Human KRT4 ELISA Kit

Alternative name
Human Cytokeratin-4 ELISA Kit;Human CK-4 ELISA Kit;Human Keratin-4 ELISA Kit;Human K4 ELISA Kit;Human Type-II keratin Kb4 ELISA Kit;Human CYK4 ELISA Kit;
Catalog
E021475
species
Human
GeneKRT4
SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 4 (KRT4). No significant cross-reactivity or interference between Keratin 4 (KRT4) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseHuman KRT4 ELISA Kit allows for the in vitro quantitative determination of KRT4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 4 (KRT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 4 (KRT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 4 (KRT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 4 (KRT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Keratin, type II cytoskeletal 4 Protein information
Uniprot ID K2C4_HUMAN
Uniprot AC P19013; F8VS64; Q6GTR8; Q96LA7; Q9BTL1;
UniGene Hs.654610; Hs.731814;
Human Keratin, type II cytoskeletal 4 Protein SEQUENCE
SEQUENCE 534 AA; 57285 MW; 3795B7C93092F97E CRC64;
MIARQQCVRG GPRGFSCGSA IVGGGKRGAF SSVSMSGGAG RCSSGGFGSR
SLYNLRGNKS ISMSVAGSRQ GACFGGAGGF GTGGFGAGGF GAGFGTGGFG
GGFGGSFSGK GGPGFPVCPA GGIQEVTINQ SLLTPLHVEI DPEIQKVRTE
EREQIKLLNN KFASFIDKVQ FLEQQNKVLE TKWNLLQQQT TTTSSKNLEP
LFETYLSVLR KQLDTLGNDK GRLQSELKTM QDSVEDFKTK YEEEINKRTA
AENDFVVLKK DVDAAYLNKV ELEAKVDSLN DEINFLKVLY DAELSQMQTH
VSDTSVVLSM DNNRNLDLDS IIAEVRAQYE EIAQRSKAEA EALYQTKVQQ
LQISVDQHGD NLKNTKSEIA ELNRMIQRLR AEIENIKKQC QTLQVSVADA
EQRGENALKD AHSKRVELEA ALQQAKEELA RMLREYQELM SVKLALDIEI
ATYRKLLEGE EYRMSGECQS AVSISVVSGS TSTGGISGGL GSGSGFGLSS
GFGSGSGSGF GFGGSVSGSS SSKIISTTTL NKRR
UCSC uc031qhk.2; human.;

Category

Human KRT4 ELISA Kit

Human Cytokeratin-4 ELISA Kit;Human CK-4 ELISA Kit;Human Keratin-4 ELISA Kit;Human K4 ELISA Kit;Human Type-II keratin Kb4 ELISA Kit;Human CYK4 ELISA Kit;