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Human KRT20 ELISA Kit

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  • Alternative name

    Human Cytokeratin-20 ELISA Kit;Human CK-20 ELISA Kit;Human Keratin-20 ELISA Kit;Human K20 ELISA Kit;Human protein IT ELISA Kit;Human CD20 ELISA Kit;Human CK20 ELISA Kit;Human KRT21 ELISA Kit;Human keratin 20 ELISA Kit;Human keratin, type I cytoskeletal 20 ELISA Kit;Human cytokeratin 20 ELISA Kit;Human keratin 20, type I ELISA Kit;
  • Catalog
    E021455
  • species
    Human
  • GeneKRT20
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CK20. No significant cross-reactivity or interference between human CK20 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CK20 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman KRT20 ELISA Kit allows for the in vitro quantitative determination of KRT20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CK20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CK20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Keratin, type I cytoskeletal 20 Protein information
  • Uniprot ID K1C20_HUMAN
  • Uniprot AC P35900; B2R6W7;
  • UniGene Hs.84905;
  • GeneID 54474
  • KEGG hsa:54474;
  • Human Keratin, type I cytoskeletal 20 Protein SEQUENCE

  • SEQUENCE 424 AA; 48487 MW; A8EF5A518C73CCE5 CRC64;

    MDFSRRSFHR SLSSSLQAPV VSTVGMQRLG TTPSVYGGAG GRGIRISNSR

    HTVNYGSDLT GGGDLFVGNE KMAMQNLNDR LASYLEKVRT LEQSNSKLEV

    QIKQWYETNA PRAGRDYSAY YRQIEELRSQ IKDAQLQNAR CVLQIDNAKL

    AAEDFRLKYE TERGIRLTVE ADLQGLNKVF DDLTLHKTDL EIQIEELNKD

    LALLKKEHQE EVDGLHKHLG NTVNVEVDAA PGLNLGVIMN EMRQKYEVMA

    QKNLQEAKEQ FERQTAVLQQ QVTVNTEELK GTEVQLTELR RTSQSLEIEL

    QSHLSMKESL EHTLEETKAR YSSQLANLQS LLSSLEAQLM QIRSNMERQN

    NEYHILLDIK TRLEQEIATY RRLLEGEDVK TTEYQLSTLE ERDIKKTRKI

    KTVVQEVVDG KVVSSEVKEV EENI

  • UCSC uc002hvl.4; human.;



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