Human KRT2 ELISA Kit

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  • Alternative name

    Human cytokeratin-2e ELISA Kit;Human CK-2e ELISA Kit;Human epithelial keratin-2e ELISA Kit;Human keratin-2 epidermis ELISA Kit;Human keratin-2e ELISA Kit;Human K2e ELISA Kit;Human type-II keratin Kb2 ELISA Kit;Human KRT2A ELISA Kit;Human KRT2E ELISA Kit;Human KRTE ELISA Kit;Human keratin 2 ELISA Kit;Human keratin, type II cytoskeletal 2 epidermal ELISA Kit;Human keratin 2, type II ELISA Kit;

  • Catalog
    E021454
  • species
    Human
  • GeneKRT2
  • Standard CurveHuman KRT2 ELISA Kit
  • Other Species Mouse Krt2 ELISA KitCanine KRT2 ELISA KitRat Krt2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 2 (KRT2). No significant cross-reactivity or interference between Keratin 2 (KRT2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman KRT2 ELISA Kit allows for the in vitro quantitative determination of KRT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 2 (KRT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 2 (KRT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 2 (KRT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 2 (KRT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Keratin, type II cytoskeletal 2 epidermal Protein information
  • Uniprot ID K22E_HUMAN
  • Uniprot AC P35908; Q4VAQ2;
  • UniGene Hs.707;
  • GeneID 3849
  • KEGG hsa:3849;
  • Human Keratin, type II cytoskeletal 2 epidermal Protein SEQUENCE
  • SEQUENCE 639 AA; 65433 MW; B80526BAF70078A7 CRC64;

    MSCQISCKSR GRGGGGGGFR GFSSGSAVVS GGSRRSTSSF SCLSRHGGGG

    GGFGGGGFGS RSLVGLGGTK SISISVAGGG GGFGAAGGFG GRGGGFGGGS

    SFGGGSGFSG GGFGGGGFGG GRFGGFGGPG GVGGLGGPGG FGPGGYPGGI

    HEVSVNQSLL QPLNVKVDPE IQNVKAQERE QIKTLNNKFA SFIDKVRFLE

    QQNQVLQTKW ELLQQMNVGT RPINLEPIFQ GYIDSLKRYL DGLTAERTSQ

    NSELNNMQDL VEDYKKKYED EINKRTAAEN DFVTLKKDVD NAYMIKVELQ

    SKVDLLNQEI EFLKVLYDAE ISQIHQSVTD TNVILSMDNS RNLDLDSIIA

    EVKAQYEEIA QRSKEEAEAL YHSKYEELQV TVGRHGDSLK EIKIEISELN

    RVIQRLQGEI AHVKKQCKNV QDAIADAEQR GEHALKDARN KLNDLEEALQ

    QAKEDLARLL RDYQELMNVK LALDVEIATY RKLLEGEECR MSGDLSSNVT

    VSVTSSTISS NVASKAAFGG SGGRGSSSGG GYSSGSSSYG SGGRQSGSRG

    GSGGGGSISG GGYGSGGGSG GRYGSGGGSK GGSISGGGYG SGGGKHSSGG

    GSRGGSSSGG GYGSGGGGSS SVKGSSGEAF GSSVTFSFR

  • UCSC uc001sat.4; human.;



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