Human KRT16 ELISA Kit

Alternative name
Human Cytokeratin-16 ELISA Kit;Human CK-16 ELISA Kit;Human Keratin-16 ELISA Kit;Human K16 ELISA Kit;Human KRT16A ELISA Kit;Human CK16 ELISA Kit;Human FNEPPK ELISA Kit;Human K1CP ELISA Kit;Human NEPPK ELISA Kit;Human PC1 ELISA Kit;Human keratin 16 ELISA Kit;Human keratin, type I cytoskeletal 16 ELISA Kit;Human cytokeratin 16 ELISA Kit;Human focal non-epidermolytic palmoplantar keratoderma ELISA Kit;Human keratin 16, type I ELISA Kit;
Catalog
E021445
species
Human
GeneKRT16
SpecificityThis assay has high sensitivity and excellent specificity for detection of KRT16. No significant cross-reactivity or interference between KRT16 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of KRT16 is typically less than 0.055ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseHuman KRT16 ELISA Kit allows for the in vitro quantitative determination of KRT16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KRT16. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to KRT16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KRT16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KRT16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Keratin, type I cytoskeletal 16 Protein information
Uniprot ID K1C16_HUMAN
Uniprot AC GLGGGYGG GFSSSSSFGS GFGGGYGGGL GAGFGGGLGA GFGGGFAGGD GLLVGSEKVT
UniGene Hs.655160;
GeneID 3868
KEGG hsa:3868;
Human Keratin, type I cytoskeletal 16 Protein SEQUENCE
SEQUENCE 473 AA; 51268 MW; BA8CE9F4716A88A4 CRC64;
MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL
SVSSRFSSGG ACGLGGGYGG GFSSSSSFGS GFGGGYGGGL GAGFGGGLGA
GFGGGFAGGD GLLVGSEKVT MQNLNDRLAS YLDKVRALEE ANADLEVKIR
DWYQRQRPSE IKDYSPYFKT IEDLRNKIIA ATIENAQPIL QIDNARLAAD
DFRTKYEHEL ALRQTVEADV NGLRRVLDEL TLARTDLEMQ IEGLKEELAY
LRKNHEEEML ALRGQTGGDV NVEMDAAPGV DLSRILNEMR DQYEQMAEKN
RRDAETWFLS KTEELNKEVA SNSELVQSSR SEVTELRRVL QGLEIELQSQ
LSMKASLENS LEETKGRYCM QLSQIQGLIG SVEEQLAQLR CEMEQQSQEY
QILLDVKTRL EQEIATYRRL LEGEDAHLSS QQASGQSYSS REVFTSSSSS
SSRQTRPILK EQSSSSFSQG QSS
UCSC uc002hxg.5; human.;

Category

Human KRT16 ELISA Kit