Human KPNA2 ELISA Kit

Alternative name
Human Karyopherin subunit alpha-2 ELISA Kit;Human RAG cohort protein 1 ELISA Kit;Human SRP1-alpha ELISA Kit;Human RCH1 ELISA Kit;Human SRP1 ELISA Kit;Human IPOA1 ELISA Kit;Human QIP2 ELISA Kit;Human SRP1alpha ELISA Kit;Human karyopherin subunit alpha 2 ELISA Kit;Human importin subunit alpha-1 ELISA Kit;Human importin subunit alpha-2 ELISA Kit;Human importin-alpha-P1 ELISA Kit;Human karyopherin alpha 2 (RAG cohort 1, importin alpha 1) ELISA Kit;Human pendulin ELISA Kit;
Catalog
E021346
species
Human
GeneKPNA2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Karyopherin Alpha 2 (KPNa2). No significant cross-reactivity or interference between Karyopherin Alpha 2 (KPNa2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 50pg/mL.
Intended UseHuman KPNA2 ELISA Kit allows for the in vitro quantitative determination of KPNA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Alpha 2 (KPNa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Karyopherin Alpha 2 (KPNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Alpha 2 (KPNa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Alpha 2 (KPNa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Importin subunit alpha-1 Protein information
Uniprot ID IMA1_HUMAN
Uniprot AC P52292; B9EJD6; Q53YE3; Q9BRU5;
UniGene Hs.594238;
GeneID 3838
KEGG hsa:3838;
Human Importin subunit alpha-1 Protein SEQUENCE
SEQUENCE 529 AA; 57862 MW; B0F94A0475B80EED CRC64;
MSTNENANTP AARLHRFKNK GKDSTEMRRR RIEVNVELRK AKKDDQMLKR
RNVSSFPDDA TSPLQENRNN QGTVNWSVDD IVKGINSSNV ENQLQATQAA
RKLLSREKQP PIDNIIRAGL IPKFVSFLGR TDCSPIQFES AWALTNIASG
TSEQTKAVVD GGAIPAFISL LASPHAHISE QAVWALGNIA GDGSVFRDLV
IKYGAVDPLL ALLAVPDMSS LACGYLRNLT WTLSNLCRNK NPAPPIDAVE
QILPTLVRLL HHDDPEVLAD TCWAISYLTD GPNERIGMVV KTGVVPQLVK
LLGASELPIV TPALRAIGNI VTGTDEQTQV VIDAGALAVF PSLLTNPKTN
IQKEATWTMS NITAGRQDQI QQVVNHGLVP FLVSVLSKAD FKTQKEAVWA
VTNYTSGGTV EQIVYLVHCG IIEPLMNLLT AKDTKIILVI LDAISNIFQA
AEKLGETEKL SIMIEECGGL DKIEALQNHE NESVYKASLS LIEKYFSVEE
EEDQNVVPET TSEGYTFQVQ DGAPGTFNF
UCSC uc002jgk.4; human.;

Category

Human KPNA2 ELISA Kit