Human SERPINA4 ELISA Kit

Alternative name
Human kallikrein inhibitor ELISA Kit;Human peptidase inhibitor 4 ELISA Kit;Human PI-4 ELISA Kit;Human serpin A4 ELISA Kit;Human KST ELISA Kit;Human PI4 ELISA Kit;Human KAL ELISA Kit;Human KLST ELISA Kit;Human kallistatin ELISA Kit;Human serpin family A member 4 ELISA Kit;Human protease inhibitor 4 (kallistatin) ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 ELISA Kit;Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 ELISA Kit;Human sserpin family A member 4 ELISA Kit;Human testicular secretory protein Li 22 ELISA Kit;
Catalog
E021331
species
Human
GeneSERPINA4
SpecificityNatural and recombinant human Kallistatin
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman SERPINA4 ELISA Kit allows for the in vitro quantitative determination of SERPINA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Kallistatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallistatin has been precoated onto 96-well plates. Standards(NSO, Q21-P427) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallistatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallistatin amount of sample captured in plate. Background: Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.
Human Kallistatin Protein information
Uniprot ID KAIN_HUMAN
Uniprot AC P29622; Q53XB5; Q86TR9; Q96BZ5;
UniGene Hs.719893;
GeneID 5267
KEGG hsa:5267;
Human Kallistatin Protein SEQUENCE
SEQUENCE 427 AA; 48542 MW; 68EBE7AF956BFB77 CRC64;
MHLIDYLLLL LVGLLALSHG QLHVEHDGES CSNSSHQQIL ETGEGSPSLK
IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ
ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK
FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE
LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ
EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR
WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG
ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN
RPFLVVIFST STQSVLFLGK VVDPTKP
UCSC uc001ydk.5; human.;

Category

Human SERPINA4 ELISA Kit