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Alternative name
Type-1 angiotensin II receptor ELISA KIT; AT1AR ELISA KIT; AT1BR ELISA KIT; Angiotensin II type-1 receptor ELISA KIT; AT1AGTR1 ELISA KIT; AGTR1A ELISA KIT; AGTR1B ELISA KIT; AT2R1 ELISA KIT; AT2R1B ELISA KIT; AT1 ELISA KIT
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Catalog
E002132
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species
Human
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GeneAT2R1
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Other Species
Human AT2R1-AA ELISA KitMouse AT2R1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of A2R1Ab. No significant cross-reactivity or interference between A2R1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between A2R1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 ng/mL.
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Intended UseHuman AT2R1 ELISA Kit allows for the in vitro quantitative determination of AT2R1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalIntended Uses: This A2R1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human A2R1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||A2R1Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Angiotensin 2 Receptor 1antigen. Standards or samples are then added to the microtiter plate wells and A2R1Ab if present, will bind to the Angiotensin 2 Receptor 1 antigen pre-coated wells. In order to quantitatively determine the amount of A2R1Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated A2R1Ab IgG is added to each well to "sandwich" the A2R1Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain A2R1Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A2R1Ab concentration in each sample is interpolated from this standard curve.
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