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Human KLK5 ELISA Kit

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  • Alternative name

    Human kallikrein-like protein 2 ELISA Kit;Human KLK-L2 ELISA Kit;Human stratum corneum tryptic enzyme ELISA Kit;Human SCTE ELISA Kit;Human KLKL2 ELISA Kit;Human kallikrein related peptidase 5 ELISA Kit;Human kallikrein-5 ELISA Kit;
  • Catalog
    E021277
  • species
    Human
  • GeneKLK5
  • SpecificityThe Human Kallikrein 5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Kallikrein 5 proteins within the range of 78 pg/ml - 5000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml
  • Intended UseHuman KLK5 ELISA Kit allows for the in vitro quantitative determination of KLK5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The Human Kallikrein 5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Kallikrein 5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Kallikrein 5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Kallikrein 5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Kallikrein 5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Kallikrein 5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14. -FOR
  • Human Kallikrein-5 Protein information
  • Uniprot ID KLK5_HUMAN
  • Uniprot AC Q9Y337; Q53ZR3; Q9HBG8;
  • UniGene Hs.50915;
  • GeneID 25818
  • KEGG hsa:25818;
  • Human Kallikrein-5 Protein SEQUENCE

  • SEQUENCE 293 AA; 32019 MW; D92C9F98055131E4 CRC64;

    MATARPPWMW VLCALITALL LGVTEHVLAN NDVSCDHPSN TVPSGSNQDL

    GAGAGEDARS DDSSSRIING SDCDMHTQPW QAALLLRPNQ LYCGAVLVHP

    QWLLTAAHCR KKVFRVRLGH YSLSPVYESG QQMFQGVKSI PHPGYSHPGH

    SNNLMLIKLN RRIRPTKDVR PINVSSHCPS AGTKCLVSGW GTTKSPQVHF

    PKVLQCLNIS VLSQKRCEDA YPRQIDDTMF CAGDKAGRDS CQGDSGGPVV

    CNGSLQGLVS WGDYPCARPN RPGVYTNLCK FTKWIQETIQ ANS

  • UCSC uc002pue.4; human.;



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