Human JAM3 ELISA Kit

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  • Alternative name

    Human JAM-C ELISA Kit;Human JAM-2 ELISA Kit;Human junctional adhesion molecule 3 ELISA Kit;Human JAM-3 ELISA Kit;Human JAMC ELISA Kit;Human junctional adhesion molecule C ELISA Kit;

  • Catalog
    E021216
  • species
    Human
  • GeneJAM3
  • Standard CurveHuman JAM3 ELISA Kit
  • Other Species Mouse Jam3 ELISA KitRat Jam3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Junctional Adhesion Molecule 3 (JAM3). No significant cross-reactivity or interference between Junctional Adhesion Molecule 3 (JAM3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.5pg/mL.
  • Intended UseHuman JAM3 ELISA Kit allows for the in vitro quantitative determination of JAM3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junctional Adhesion Molecule 3 (JAM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junctional Adhesion Molecule 3 (JAM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junctional Adhesion Molecule 3 (JAM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Junctional Adhesion Molecule 3 (JAM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Junctional adhesion molecule C Protein information
  • Uniprot ID JAM3_HUMAN
  • Uniprot AC Q9BX67; B3KWG9; Q8WWL8; Q96FL1;
  • UniGene Hs.150718;
  • GeneID 83700
  • KEGG hsa:83700;
  • Human Junctional adhesion molecule C Protein SEQUENCE
  • SEQUENCE 310 AA; 35020 MW; CE39ADF33EA1DAB9 CRC64;

    MALRRPPRLR LCARLPDFFL LLLFRGCLIG AVNLKSSNRT PVVQEFESVE

    LSCIITDSQT SDPRIEWKKI QDEQTTYVFF DNKIQGDLAG RAEILGKTSL

    KIWNVTRRDS ALYRCEVVAR NDRKEIDEIV IELTVQVKPV TPVCRVPKAV

    PVGKMATLHC QESEGHPRPH YSWYRNDVPL PTDSRANPRF RNSSFHLNSE

    TGTLVFTAVH KDDSGQYYCI ASNDAGSARC EEQEMEVYDL NIGGIIGGVL

    VVLAVLALIT LGICCAYRRG YFINNKQDGE SYKNPGKPDG VNYIRTDEEG

    DFRHKSSFVI

  • UCSC uc001qhb.4; human. [Q9BX67-1];



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