Human PLG ELISA Kit

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  • Alternative name

    Human plasminogen ELISA Kit;Human plasmin ELISA Kit;

  • Catalog
    E002116
  • species
    Human
  • GenePLG
  • Standard CurveHuman PLG ELISA Kit
  • Other Species Mouse Plg ELISA KitBovine PLG ELISA KitCanine PLG ELISA KitGoat PLG ELISA KitHorse PLG ELISA KitPorcine PLG ELISA KitRat Plg ELISA KitSheep PLG ELISA Kit
  • SpecificityNatural and recombinant human Angiostatin K1-3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 50pg/ml
  • Intended UseHuman PLG ELISA Kit allows for the in vitro quantitative determination of PLG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human Angiostatin K1-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiostatin K1-3 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiostatin K1-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiostatin K1-3 amount of sample captured in plate. Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED50 =70 nM) relative to kringles 1-4 (ED50 = 135 nM).
  • Human Plasmin light chain B Protein information
  • Uniprot ID PLMN_HUMAN
  • Uniprot AC P00747; Q15146; Q5TEH4; Q6PA00;
  • UniGene Hs.143436;
  • GeneID 5340
  • KEGG hsa:5340;
  • Human Plasmin light chain B Protein SEQUENCE
  • SEQUENCE 810 AA; 90569 MW; 8B31CB877CCB3AB6 CRC64;

    MEHKEVVLLL LLFLKSGQGE PLDDYVNTQG ASLFSVTKKQ LGAGSIEECA

    AKCEEDEEFT CRAFQYHSKE QQCVIMAENR KSSIIIRMRD VVLFEKKVYL

    SECKTGNGKN YRGTMSKTKN GITCQKWSST SPHRPRFSPA THPSEGLEEN

    YCRNPDNDPQ GPWCYTTDPE KRYDYCDILE CEEECMHCSG ENYDGKISKT

    MSGLECQAWD SQSPHAHGYI PSKFPNKNLK KNYCRNPDRE LRPWCFTTDP

    NKRWELCDIP RCTTPPPSSG PTYQCLKGTG ENYRGNVAVT VSGHTCQHWS

    AQTPHTHNRT PENFPCKNLD ENYCRNPDGK RAPWCHTTNS QVRWEYCKIP

    SCDSSPVSTE QLAPTAPPEL TPVVQDCYHG DGQSYRGTSS TTTTGKKCQS

    WSSMTPHRHQ KTPENYPNAG LTMNYCRNPD ADKGPWCFTT DPSVRWEYCN

    LKKCSGTEAS VVAPPPVVLL PDVETPSEED CMFGNGKGYR GKRATTVTGT

    PCQDWAAQEP HRHSIFTPET NPRAGLEKNY CRNPDGDVGG PWCYTTNPRK

    LYDYCDVPQC AAPSFDCGKP QVEPKKCPGR VVGGCVAHPH SWPWQVSLRT

    RFGMHFCGGT LISPEWVLTA AHCLEKSPRP SSYKVILGAH QEVNLEPHVQ

    EIEVSRLFLE PTRKDIALLK LSSPAVITDK VIPACLPSPN YVVADRTECF

    ITGWGETQGT FGAGLLKEAQ LPVIENKVCN RYEFLNGRVQ STELCAGHLA

    GGTDSCQGDS GGPLVCFEKD KYILQGVTSW GLGCARPNKP GVYVRVSRFV

    TWIEGVMRNN

  • UCSC uc003qtm.5; human.;




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