Human IZUMO1 ELISA Kit

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  • Alternative name

    Human oocyte binding/fusion factor ELISA Kit;Human OBF ELISA Kit;Human sperm-specific protein Izumo ELISA Kit;Human IZUMO ELISA Kit;Human izumo sperm-egg fusion 1 ELISA Kit;Human izumo sperm-egg fusion protein 1 ELISA Kit;

  • Catalog
    E021149
  • species
    Human
  • GeneIZUMO1
  • Other Species Mouse Izumo1 ELISA KitRat Izumo1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4pg/ml
  • Intended UseHuman IZUMO1 ELISA Kit allows for the in vitro quantitative determination of IZUMO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IZUMO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IZUMO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IZUMO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IZUMO1 can be calculated.
  • Human Izumo sperm-egg fusion protein 1 Protein information
  • Uniprot ID IZUM1_HUMAN
  • Uniprot AC Q8IYV9; Q6Q8P6; Q6Q8P7;
  • UniGene Hs.400688;
  • GeneID 284359
  • KEGG hsa:284359;
  • Human Izumo sperm-egg fusion protein 1 Protein SEQUENCE
  • SEQUENCE 350 AA; 38930 MW; 8741BB3F93ABCFA9 CRC64;

    MGPHFTLLCA ALAGCLLPAE GCVICDPSVV LALKSLEKDY LPGHLDAKHH

    KAMMERVENA VKDFQELSLN EDAYMGVVDE ATLQKGSWSL LKDLKRITDS

    DVKGDLFVKE LFWMLHLQKE TFATYVARFQ KEAYCPNKCG VMLQTLIWCK

    NCKKEVHACR KSYDCGERNV EVPQMEDMIL DCELNWHQAS EGLTDYSFYR

    VWGNNTETLV SKGKEATLTK PMVGPEDAGS YRCELGSVNS SPATIINFHV

    TVLPKMIKEE KPSPNIVTPG EATTESSISL QPLQPEKMLA SRLLGLLICG

    SLALITGLTF AIFRRRKVID FIKSSLFGLG SGAAEQTQVP KEKATDSRQQ

  • UCSC uc002pkj.3; human. [Q8IYV9-1];



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