SpecificityThis assay has high sensitivity and excellent specificity for detection of ITG alphaVbeta3 . No significant cross-reactivity or interference between ITG alphaVbeta3 and analogues was observed.
Intended UseHuman ITG ELISA Kit allows for the in vitro quantitative determination of ITG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ITG alpha Vbeta3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ITG alphaVbeta3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ITG alphaVbeta3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ITG alphaV beta3 can be calculated.