Human IDH1 ELISA Kit

Alternative name
Human IDH ELISA Kit;Human Cytosolic NADP-isocitrate dehydrogenase ELISA Kit;Human IDP ELISA Kit;Human NADP(+)-specific ICDH ELISA Kit;Human oxalosuccinate decarboxylase ELISA Kit;Human PICD ELISA Kit;Human HEL-216 ELISA Kit;Human HEL-S-26 ELISA Kit;Human IDCD ELISA Kit;Human IDPC ELISA Kit;Human isocitrate dehydrogenase (NADP(+)) 1, cytosolic ELISA Kit;Human isocitrate dehydrogenase [NADP] cytoplasmic ELISA Kit;Human NADP-dependent isocitrate dehydrogenase, cytosolic ELISA Kit;Human NADP-dependent isocitrate dehydrogenase, peroxisomal ELISA Kit;Human epididymis luminal protein 216 ELISA Kit;Human epididymis secretory protein Li 26 ELISA Kit;Human isocitrate dehydrogenase 1 (NADP+), soluble ELISA Kit;
Catalog
E021121
species
Human
GeneIDH1
Standard Curve
Other Species Mouse Idh1 ELISA Kit Bovine IDH1 ELISA Kit Porcine IDH1 ELISA Kit Rat Idh1 ELISA Kit Sheep IDH1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 1.39ng/mL.
Intended UseHuman IDH1 ELISA Kit allows for the in vitro quantitative determination of IDH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isocitrate Dehydrogenase 1, Soluble (IDH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isocitrate Dehydrogenase 1, Soluble (IDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Isocitrate dehydrogenase [NADP] cytoplasmic Protein information
Uniprot ID IDHC_HUMAN
Uniprot AC O75874; Q567U4; Q6FHQ6; Q7Z3V0; Q93090; Q9NTJ9; Q9UKW8;
UniGene Hs.593422;
GeneID 3417
KEGG hsa:3417;
Human Isocitrate dehydrogenase [NADP] cytoplasmic Protein SEQUENCE
SEQUENCE 414 AA; 46659 MW; 60428B0B6E5851DC CRC64;
MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD
ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG
KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA
LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK
ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
LGENLKIKLA QAKL

Related products with Human IDH1 ELISA Kit

Category

Human IDH1 ELISA Kit