Human ICA1 ELISA Kit

Alternative name
Human 69 kDa islet cell autoantigen ELISA Kit;Human ICA69 ELISA Kit;Human islet cell autoantigen p69 ELISA Kit;Human ICAp69 ELISA Kit;Human p69 ELISA Kit;Human islet cell autoantigen 1 ELISA Kit;Human diabetes mellitus type I autoantigen ELISA Kit;Human islet cell autoantigen 1 isoform ELISA Kit;Human islet cell autoantigen 1, 69kDa ELISA Kit;Human testicular tissue protein Li 162 ELISA Kit;
Catalog
E021105
species
Human
GeneICA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Islet Cell Autoantigen 1 (ICA1). No significant cross-reactivity or interference between Islet Cell Autoantigen 1 (ICA1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman ICA1 ELISA Kit allows for the in vitro quantitative determination of ICA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Islet Cell Autoantigen 1 (ICA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Islet Cell Autoantigen 1 (ICA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Islet Cell Autoantigen 1 (ICA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Islet Cell Autoantigen 1 (ICA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Islet cell autoantigen 1 Protein information
Uniprot ID ICA69_HUMAN
Uniprot AC Q05084; A8K7U1; B3FTQ2; P78506; Q13824; Q96HG3;
UniGene Hs.487561;
GeneID 3382
KEGG hsa:3382;
Human Islet cell autoantigen 1 Protein SEQUENCE
SEQUENCE 483 AA; 54645 MW; 1696F928EE7454C9 CRC64;
MSGHKCSYPW DLQDRYAQDK SVVNKMQQKY WETKQAFIKA TGKKEDEHVV
ASDADLDAKL ELFHSIQRTC LDLSKAIVLY QKRICFLSQE ENELGKFLRS
QGFQDKTRAG KMMQATGKAL CFSSQQRLAL RNPLCRFHQE VETFRHRAIS
DTWLTVNRME QCRTEYRGAL LWMKDVSQEL DPDLYKQMEK FRKVQTQVRL
AKKNFDKLKM DVCQKVDLLG ASRCNLLSHM LATYQTTLLH FWEKTSHTMA
AIHESFKGYQ PYEFTTLKSL QDPMKKLVEK EEKKKINQQE STDAAVQEPS
QLISLEEENQ RKESSSFKTE DGKSILSALD KGSTHTACSG PIDELLDMKS
EEGACLGPVA GTPEPEGADK DDLLLLSEIF NASSLEEGEF SKEWAAVFGD
GQVKEPVPTM ALGEPDPKAQ TGSGFLPSQL LDQNMKDLQA SLQEPAKAAS
DLTAWFSLFA DLDPLSNPDA VGKTDKEHEL LNA
UCSC uc003srm.4; human. [Q05084-1];

Category

Human ICA1 ELISA Kit