Human ICA1 ELISA Kit

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  • Alternative name

    Human 69 kDa islet cell autoantigen ELISA Kit;Human ICA69 ELISA Kit;Human islet cell autoantigen p69 ELISA Kit;Human ICAp69 ELISA Kit;Human p69 ELISA Kit;Human islet cell autoantigen 1 ELISA Kit;Human diabetes mellitus type I autoantigen ELISA Kit;Human islet cell autoantigen 1 isoform ELISA Kit;Human islet cell autoantigen 1, 69kDa ELISA Kit;Human testicular tissue protein Li 162 ELISA Kit;

  • Catalog
    E021105
  • species
    Human
  • GeneICA1
  • Standard CurveHuman ICA1 ELISA Kit
  • Other Species Mouse Ica1 ELISA KitRat Ica1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Islet Cell Autoantigen 1 (ICA1). No significant cross-reactivity or interference between Islet Cell Autoantigen 1 (ICA1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseHuman ICA1 ELISA Kit allows for the in vitro quantitative determination of ICA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Islet Cell Autoantigen 1 (ICA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Islet Cell Autoantigen 1 (ICA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Islet Cell Autoantigen 1 (ICA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Islet Cell Autoantigen 1 (ICA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Islet cell autoantigen 1 Protein information
  • Uniprot ID ICA69_HUMAN
  • Uniprot AC Q05084; A8K7U1; B3FTQ2; P78506; Q13824; Q96HG3;
  • UniGene Hs.487561;
  • GeneID 3382
  • KEGG hsa:3382;
  • Human Islet cell autoantigen 1 Protein SEQUENCE
  • SEQUENCE 483 AA; 54645 MW; 1696F928EE7454C9 CRC64;

    MSGHKCSYPW DLQDRYAQDK SVVNKMQQKY WETKQAFIKA TGKKEDEHVV

    ASDADLDAKL ELFHSIQRTC LDLSKAIVLY QKRICFLSQE ENELGKFLRS

    QGFQDKTRAG KMMQATGKAL CFSSQQRLAL RNPLCRFHQE VETFRHRAIS

    DTWLTVNRME QCRTEYRGAL LWMKDVSQEL DPDLYKQMEK FRKVQTQVRL

    AKKNFDKLKM DVCQKVDLLG ASRCNLLSHM LATYQTTLLH FWEKTSHTMA

    AIHESFKGYQ PYEFTTLKSL QDPMKKLVEK EEKKKINQQE STDAAVQEPS

    QLISLEEENQ RKESSSFKTE DGKSILSALD KGSTHTACSG PIDELLDMKS

    EEGACLGPVA GTPEPEGADK DDLLLLSEIF NASSLEEGEF SKEWAAVFGD

    GQVKEPVPTM ALGEPDPKAQ TGSGFLPSQL LDQNMKDLQA SLQEPAKAAS

    DLTAWFSLFA DLDPLSNPDA VGKTDKEHEL LNA

  • UCSC uc003srm.4; human. [Q05084-1];



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