Human ISG15 ELISA Kit

Alternative name
Human Interferon-induced 15 kDa protein ELISA Kit;Human Interferon-induced 17 kDa protein ELISA Kit;Human IP17 ELISA Kit;Human ubiquitin cross-reactive protein ELISA Kit;Human hUCRP ELISA Kit;Human G1P2 ELISA Kit;Human UCRP ELISA Kit;Human IFI15 ELISA Kit;Human IMD38 ELISA Kit;Human ISG15 ubiquitin-like modifier ELISA Kit;Human ubiquitin-like protein ISG15 ELISA Kit;Human interferon, alpha-inducible protein (clone IFI-15K) ELISA Kit;Human interferon-induced 17-kDa/15-kDa protein ELISA Kit;Human interferon-stimulated protein, 15 kDa ELISA Kit;
Catalog
E021086
species
Human
GeneISG15
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ISG15. No significant cross-reactivity or interference between human ISG15 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ISG15 is typically less than 0.195 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman ISG15 ELISA Kit allows for the in vitro quantitative determination of ISG15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ISG15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ISG15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ISG15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ISG15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Ubiquitin-like protein ISG15 Protein information
Uniprot ID ISG15_HUMAN
Uniprot AC P05161; Q5SVA4; Q7Z2G2; Q96GF0;
UniGene Hs.458485;
GeneID 9636
KEGG hsa:9636;
Human Ubiquitin-like protein ISG15 Protein SEQUENCE
SEQUENCE 165 AA; 17888 MW; B6858A15AB0FFFDE CRC64;
MGWDLTVKML AGNEFQVSLS SSMSVSELKA QITQKIGVHA FQQRLAVHPS
GVALQDRVPL ASQGLGPGST VLLVVDKCDE PLSILVRNNK GRSSTYEVRL
TQTVAHLKQQ VSGLEGVQDD LFWLTFEGKP LEDQLPLGEY GLKPLSTVFM
NLRLRGGGTE PGGRS
UCSC uc001acj.5; human.;

Human ISG15 ELISA Kit

Contact Us