SensitivityThe minimum detectable dose of human ISG15 is typically less than 0.195 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman ISG15 ELISA Kit allows for the in vitro quantitative determination of ISG15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ISG15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ISG15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ISG15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ISG15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Ubiquitin-like protein ISG15 Protein information
P05161; Q5SVA4; Q7Z2G2; Q96GF0;
Human Ubiquitin-like protein ISG15 Protein SEQUENCE