Human IVL ELISA Kit

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  • Alternative name

    Human involucrin ELISA Kit;

  • Catalog
    E021017
  • species
    Human
  • GeneIVL
  • Standard CurveHuman IVL ELISA Kit
  • Other Species Mouse Ivl ELISA KitCanine IVL ELISA KitPorcine IVL ELISA KitRat Ivl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human IVL. No significant cross-reactivity or interference between Human IVL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.31 ng/ml
  • Intended UseHuman IVL ELISA Kit allows for the in vitro quantitative determination of IVL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. In humans, Involucrin is encoded by the IVL gene. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. Involucrin is synthesised in the stratum spinosum and cross linked in the stratum granulosum by the transglutaminase enzyme that makes it highly stable. Thus it provide structural support to the cell, thereby allowing the cell to resist invasion by micro-organism. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to INV. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for INV and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain INV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The 3 enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of INV in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Involucrin Protein information
  • Uniprot ID INVO_HUMAN
  • Uniprot AC P07476; Q5T7P4;
  • UniGene Hs.516439;
  • GeneID 3713
  • KEGG hsa:3713;
  • Human Involucrin Protein SEQUENCE
  • SEQUENCE 585 AA; 68479 MW; 775D2AFB90F647E8 CRC64;

    MSQQHTLPVT LSPALSQELL KTVPPPVNTH QEQMKQPTPL PPPCQKVPVE

    LPVEVPSKQE EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK

    AENPEQQLKQ EKTQRDQQLN KQLEEEKKLL DQQLDQELVK RDEQLGMKKE

    QLLELPEQQE GHLKHLEQQE GQLKHPEQQE GQLELPEQQE GQLELPEQQE

    GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPQQQE GQLELSEQQE

    GQLELSEQQE GQLKHLEHQE GQLEVPEEQM GQLKYLEQQE GQLKHLDQQE

    KQPELPEQQM GQLKHLEQQE GQPKHLEQQE GQLEQLEEQE GQLKHLEQQE

    GQLEHLEHQE GQLGLPEQQV LQLKQLEKQQ GQPKHLEEEE GQLKHLVQQE

    GQLKHLVQQE GQLEQQERQV EHLEQQVGQL KHLEEQEGQL KHLEQQQGQL

    EVPEQQVGQP KNLEQEEKQL ELPEQQEGQV KHLEKQEAQL ELPEQQVGQP

    KHLEQQEKHL EHPEQQDGQL KHLEQQEGQL KDLEQQKGQL EQPVFAPAPG

    QVQDIQPALP TKGEVLLPVE HQQQKQEVQW PPKHK

  • UCSC uc001fau.4; human.;


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