Human IVL ELISA Kit

Alternative name
Human involucrin ELISA Kit;
Catalog
E021017
species
Human
GeneIVL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human IVL. No significant cross-reactivity or interference between Human IVL and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.31 ng/ml
Intended UseHuman IVL ELISA Kit allows for the in vitro quantitative determination of IVL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. In humans, Involucrin is encoded by the IVL gene. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. Involucrin is synthesised in the stratum spinosum and cross linked in the stratum granulosum by the transglutaminase enzyme that makes it highly stable. Thus it provide structural support to the cell, thereby allowing the cell to resist invasion by micro-organism. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to INV. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for INV and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain INV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The 3 enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of INV in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Involucrin Protein information
Uniprot ID INVO_HUMAN
Uniprot AC P07476; Q5T7P4;
UniGene Hs.516439;
GeneID 3713
KEGG hsa:3713;
Human Involucrin Protein SEQUENCE
SEQUENCE 585 AA; 68479 MW; 775D2AFB90F647E8 CRC64;
MSQQHTLPVT LSPALSQELL KTVPPPVNTH QEQMKQPTPL PPPCQKVPVE
LPVEVPSKQE EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK
AENPEQQLKQ EKTQRDQQLN KQLEEEKKLL DQQLDQELVK RDEQLGMKKE
QLLELPEQQE GHLKHLEQQE GQLKHPEQQE GQLELPEQQE GQLELPEQQE
GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPQQQE GQLELSEQQE
GQLELSEQQE GQLKHLEHQE GQLEVPEEQM GQLKYLEQQE GQLKHLDQQE
KQPELPEQQM GQLKHLEQQE GQPKHLEQQE GQLEQLEEQE GQLKHLEQQE
GQLEHLEHQE GQLGLPEQQV LQLKQLEKQQ GQPKHLEEEE GQLKHLVQQE
GQLKHLVQQE GQLEQQERQV EHLEQQVGQL KHLEEQEGQL KHLEQQQGQL
EVPEQQVGQP KNLEQEEKQL ELPEQQEGQV KHLEKQEAQL ELPEQQVGQP
KHLEQQEKHL EHPEQQDGQL KHLEQQEGQL KDLEQQKGQL EQPVFAPAPG
QVQDIQPALP TKGEVLLPVE HQQQKQEVQW PPKHK
UCSC uc001fau.4; human.;

Category

Human IVL ELISA Kit

Human involucrin ELISA Kit;