Human ADAM22 ELISA Kit

Alternative name
Human ADAM 22 ELISA Kit;Human metalloproteinase-disintegrin ADAM22-3 ELISA Kit;Human metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2 ELISA Kit;Human MDC2 ELISA Kit;Human ADAM metallopeptidase domain 22 ELISA Kit;Human disintegrin and metalloproteinase domain-containing protein 22 ELISA Kit;Human a disintegrin and metalloproteinase domain 22 ELISA Kit;
Catalog
E000021
species
Human
GeneADAM22
Standard Curve
Other Species Mouse Adam22 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 22 (ADAM22). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 22 (ADAM22) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman ADAM22 ELISA Kit allows for the in vitro quantitative determination of ADAM22 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 22 (ADAM22). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 22 (ADAM22). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 22 (ADAM22), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 22 (ADAM22) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Disintegrin and metalloproteinase domain-containing protein 22 Protein information
Uniprot ID ADA22_HUMAN
Uniprot AC Q9P0K1; O75075; O75076; Q9P0K2; Q9UIA1; Q9UKK2;
UniGene Hs.256398;
GeneID 53616
KEGG hsa:53616;
Human Disintegrin and metalloproteinase domain-containing protein 22 Protein SEQUENCE
SEQUENCE 906 AA; 100433 MW; 265ECCD0FA6C088B CRC64;
MQAAVAVSVP FLLLCVLGTC PPARCGQAGD ASLMELEKRK ENRFVERQSI
VPLRLIYRSG GEDESRHDAL DTRVRGDLGG PQLTHVDQAS FQVDAFGTSF
ILDVVLNHDL LSSEYIERHI EHGGKTVEVK GGEHCYYQGH IRGNPDSFVA
LSTCHGLHGM FYDGNHTYLI EPEENDTTQE DFHFHSVYKS RLFEFSLDDL
PSEFQQVNIT PSKFILKPRP KRSKRQLRRY PRNVEEETKY IELMIVNDHL
MFKKHRLSVV HTNTYAKSVV NMADLIYKDQ LKTRIVLVAM ETWATDNKFA
ISENPLITLR EFMKYRRDFI KEKSDAVHLF SGSQFESSRS GAAYIGGICS
LLKGGGVNEF GKTDLMAVTL AQSLAHNIGI ISDKRKLASG ECKCEDTWSG
CIMGDTGYYL PKKFTQCNIE EYHDFLNSGG GACLFNKPSK LLDPPECGNG
FIETGEECDC GTPAECVLEG AECCKKCTLT QDSQCSDGLC CKKCKFQPMG
TVCREAVNDC DIRETCSGNS SQCAPNIHKM DGYSCDGVQG ICFGGRCKTR
DRQCKYIWGQ KVTASDKYCY EKLNIEGTEK GNCGKDKDTW IQCNKRDVLC
GYLLCTNIGN IPRLGELDGE ITSTLVVQQG RTLNCSGGHV KLEEDVDLGY
VEDGTPCGPQ MMCLEHRCLP VASFNFSTCL SSKEGTICSG NGVCSNELKC
VCNRHWIGSD CNTYFPHNDD AKTGITLSGN GVAGTNIIIG IIAGTILVLA
LILGITAWGY KNYREQRQLP QGDYVKKPGD GDSFYSDIPP GVSTNSASSS
KKRSNGLSHS WSERIPDTKH ISDICENGRP RSNSWQGNLG GNKKKIRGKR
FRPRSNSTET LSPAKSPSSS TGSIASSRKY PYPMPPLPDE DKKVNRQSAR
LWETSI
UCSC uc003ujk.3; human. [Q9P0K1-1];

Category

Human ADAM22 ELISA Kit