Human IL-18R1/IL-18R ELISA Kit

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  • Alternative name

    Interleukin-18 receptor 1 ELISA KIT; CD218 antigen-like family member A ELISA KIT; CDw218a ELISA KIT; IL1 receptor-related protein ELISA KIT; IL-1Rrp ELISA KIT; IL1R-rp ELISA KIT; CD_antigen: CD218aIL18R1 ELISA KIT; IL1RRP ELISA KIT; IL-18R-1 ELISA KIT; IL-18R1 ELISA KIT; IL-1Rrp ELISA KIT; IL1R-rp ELISA KIT

  • Catalog
  • species
  • GeneIL-18R1/IL-18R
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<18.75pg/ml
  • Intended UseHuman IL-18R1/IL-18R ELISA Kit allows for the in vitro quantitative determination of IL-18R1/IL-18R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IL-18R1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IL-18R1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-18R1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IL-18R1 can be calculated.

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