Human ANGPTL5 ELISA Kit

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  • Alternative name

    Human angiopoietin-like protein 5 ELISA Kit;Human angiopoietin like 5 ELISA Kit;Human angiopoietin-related protein 5 ELISA Kit;

  • Catalog
    E002075
  • species
    Human
  • GeneANGPTL5
  • Standard CurveHuman ANGPTL5 ELISA Kit
  • Other Species Mouse ANGPTL5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ANGPTL5. No significant cross-reactivity or interference between ANGPTL5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANGPTL5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ANGPTL5 ELISA Kit allows for the in vitro quantitative determination of ANGPTL5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Intended Uses||This ANGPTL5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANGPTL5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ANGPTL5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANGPTL5 antibody and an ANGPTL5-HRP conjugate. The assay sample and buffer are incubated together with ANGPTL5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANGPTL5 concentration since ANGPTL5 from samples and ANGPTL5-HRP conjugate compete for the anti-ANGPTL5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANGPTL5 from the sample, fewer sites are left to bind ANGPTL5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANGPTL5 concentration in each sample is interpolated from this standard curve.
  • Human Angiopoietin-related protein 5 Protein information
  • Uniprot ID ANGL5_HUMAN
  • Uniprot AC Q86XS5; A8K658; Q86VR9;
  • UniGene Hs.318370;
  • GeneID 253935
  • KEGG hsa:253935;
  • Human Angiopoietin-related protein 5 Protein SEQUENCE
  • SEQUENCE 388 AA; 44144 MW; 4060C998885151BF CRC64;

    MMSPSQASLL FLNVCIFICG EAVQGNCVHH STDSSVVNIV EDGSNAKDES

    KSNDTVCKED CEESCDVKTK ITREEKHFMC RNLQNSIVSY TRSTKKLLRN

    MMDEQQASLD YLSNQVNELM NRVLLLTTEV FRKQLDPFPH RPVQSHGLDC

    TDIKDTIGSV TKTPSGLYII HPEGSSYPFE VMCDMDYRGG GRTVIQKRID

    GIIDFQRLWC DYLDGFGDLL GEFWLGLKKI FYIVNQKNTS FMLYVALESE

    DDTLAYASYD NFWLEDETRF FKMHLGRYSG NAGDAFRGLK KEDNQNAMPF

    STSDVDNDGC RPACLVNGQS VKSCSHLHNK TGWWFNECGL ANLNGIHHFS

    GKLLATGIQW GTWTKNNSPV KIKSVSMKIR RMYNPYFK

  • UCSC uc001pgl.3; human.;


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