Human IL 32a ELISA Kit

Contact Us
  • Alternative name

    Interleukin-32 ELISA KIT; Natural killer cells protein 4 ELISA KIT; Tumor necrosis factor alpha-inducing factorIL32 ELISA KIT; NK4 ELISA KIT; TAIF ELISA KIT; IL-32 ELISA KIT

  • Catalog
    E020715
  • species
    Human
  • GeneIL 32a
  • Other Species Mouse IL-32a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of IL-32alpha. No significant cross-reactivity or interference between IL-32alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-32alpha and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman IL 32a ELISA Kit allows for the in vitro quantitative determination of IL 32a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: IL-32alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-32alpha antibody and an IL-32alpha-HRP conjugate. The assay sample and buffer are incubated together with IL-32alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-32alpha concentration since IL-32alpha from samples and IL-32alpha-HRP conjugate compete for the anti-IL-32alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-32alpha from the sample, fewer sites are left to bind IL-32alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-32alpha concentration in each sample is interpolated from this standard curve.


Related products with Human IL 32a ELISA Kit

Inquiry About Human IL 32a ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!