Human IL3RA ELISA Kit

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  • Alternative name

    Human IL-3 receptor subunit alpha ELISA Kit;Human IL-3R subunit alpha ELISA Kit;Human IL-3R-alpha ELISA Kit;Human IL-3RA ELISA Kit;Human IL3R ELISA Kit;Human CD123 ELISA Kit;Human IL3RAY ELISA Kit;Human IL3RX ELISA Kit;Human IL3RY ELISA Kit;Human hIL-3Ra ELISA Kit;Human interleukin 3 receptor subunit alpha ELISA Kit;Human interleukin-3 receptor subunit alpha ELISA Kit;Human CD123 antigen ELISA Kit;Human IL-3 receptor alpha SP2 isoform ELISA Kit;Human interleukin 3 receptor, alpha (low affinity) ELISA Kit;

  • Catalog
    E020701
  • species
    Human
  • GeneIL3RA
  • Standard CurveHuman IL3RA ELISA Kit
  • Other Species Mouse Il3ra ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 3 Receptor Alpha (IL3Ra). No significant cross-reactivity or interference between Interleukin 3 Receptor Alpha (IL3Ra) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.118ng/mL.
  • Intended UseHuman IL3RA ELISA Kit allows for the in vitro quantitative determination of IL3RA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 3 Receptor Alpha (IL3Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 3 Receptor Alpha (IL3Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 3 Receptor Alpha (IL3Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 3 Receptor Alpha (IL3Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Interleukin-3 receptor subunit alpha Protein information
  • Uniprot ID IL3RA_HUMAN
  • Uniprot AC P26951; A8K3F3; B9VI81; Q5HYQ7; Q5HYQ8; Q9UEH7;
  • UniGene Hs.632790;
  • GeneID 3563
  • KEGG hsa:3563;
  • Human Interleukin-3 receptor subunit alpha Protein SEQUENCE
  • SEQUENCE 378 AA; 43330 MW; 716CE1803F2E5FC0 CRC64;

    MVLLWLTLLL IALPCLLQTK EDPNPPITNL RMKAKAQQLT WDLNRNVTDI

    ECVKDADYSM PAVNNSYCQF GAISLCEVTN YTVRVANPPF STWILFPENS

    GKPWAGAENL TCWIHDVDFL SCSWAVGPGA PADVQYDLYL NVANRRQQYE

    CLHYKTDAQG TRIGCRFDDI SRLSSGSQSS HILVRGRSAA FGIPCTDKFV

    VFSQIEILTP PNMTAKCNKT HSFMHWKMRS HFNRKFRYEL QIQKRMQPVI

    TEQVRDRTSF QLLNPGTYTV QIRARERVYE FLSAWSTPQR FECDQEEGAN

    TRAWRTSLLI ALGTLLALVC VFVICRRYLV MQRLFPRIPH MKDPIGDSFQ

    NDKLVVWEAG KAGLEECLVT EVQVVQKT

  • UCSC uc004cps.4; human. [P26951-1];


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