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Human ACE ELISA Kit

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  • Alternative name

    Human ACE ELISA Kit;Human dipeptidyl carboxypeptidase I ELISA Kit;Human kininase II ELISA Kit;Human DCP ELISA Kit;Human DCP1 ELISA Kit;Human ACE1 ELISA Kit;Human CD143 ELISA Kit;Human angiotensin I converting enzyme ELISA Kit;Human angiotensin-converting enzyme ELISA Kit;Human CD143 antigen ELISA Kit;Human angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 ELISA Kit;Human angiotensin converting enzyme, somatic isoform ELISA Kit;Human carboxycathepsin ELISA Kit;Human dipeptidyl carboxypeptidase 1 ELISA Kit;Human peptidase P ELISA Kit;
  • Catalog
    E000207
  • species
    Human
  • GeneACE
  • Standard CurveHuman ACE ELISA Kit
  • Other Species Human ACE I ELISA KitHuman ACE II ELISA KitMouse Ace ELISA KitMouse ACE I ELISA KitMouse ACE II ELISA KitBovine ACE ELISA KitChicken ACE ELISA KitRabbit ACE ELISA KitRat Ace ELISA Kit
  • SpecificityNatural and recombinant human ACE
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 5pg/ml
  • Intended UseHuman ACE ELISA Kit allows for the in vitro quantitative determination of ACE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ACE has been precoated onto 96-well plates. Standards(NSO, L30-L1261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ACE amount of sample captured in plate. Background: Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073.1 Although ACE has been studied primarily in the contextof its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm.2 The standard product used in this kit is recombinanthuman ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
  • Human Angiotensin-converting enzyme, soluble form Protein information
  • Uniprot ID ACE_HUMAN
  • Uniprot AC P12821; B0LPF0; B4DXI3; E7EU16; P22966; Q53YX9; Q59GY8; Q7M4L4;
  • UniGene Hs.298469;
  • GeneID 1636
  • KEGG hsa:1636;
  • Human Angiotensin-converting enzyme, soluble form Protein SEQUENCE

  • SEQUENCE 1306 AA; 149715 MW; 1B33BCA7301A26AA CRC64;

    MGAASGRRGP GLLLPLPLLL LLPPQPALAL DPGLQPGNFS ADEAGAQLFA

    QSYNSSAEQV LFQSVAASWA HDTNITAENA RRQEEAALLS QEFAEAWGQK

    AKELYEPIWQ NFTDPQLRRI IGAVRTLGSA NLPLAKRQQY NALLSNMSRI

    YSTAKVCLPN KTATCWSLDP DLTNILASSR SYAMLLFAWE GWHNAAGIPL

    KPLYEDFTAL SNEAYKQDGF TDTGAYWRSW YNSPTFEDDL EHLYQQLEPL

    YLNLHAFVRR ALHRRYGDRY INLRGPIPAH LLGDMWAQSW ENIYDMVVPF

    PDKPNLDVTS TMLQQGWNAT HMFRVAEEFF TSLELSPMPP EFWEGSMLEK

    PADGREVVCH ASAWDFYNRK DFRIKQCTRV TMDQLSTVHH EMGHIQYYLQ

    YKDLPVSLRR GANPGFHEAI GDVLALSVST PEHLHKIGLL DRVTNDTESD

    INYLLKMALE KIAFLPFGYL VDQWRWGVFS GRTPPSRYNF DWWYLRTKYQ

    GICPPVTRNE THFDAGAKFH VPNVTPYIRY FVSFVLQFQF HEALCKEAGY

    EGPLHQCDIY RSTKAGAKLR KVLQAGSSRP WQEVLKDMVG LDALDAQPLL

    KYFQPVTQWL QEQNQQNGEV LGWPEYQWHP PLPDNYPEGI DLVTDEAEAS

    KFVEEYDRTS QVVWNEYAEA NWNYNTNITT ETSKILLQKN MQIANHTLKY

    GTQARKFDVN QLQNTTIKRI IKKVQDLERA ALPAQELEEY NKILLDMETT

    YSVATVCHPN GSCLQLEPDL TNVMATSRKY EDLLWAWEGW RDKAGRAILQ

    FYPKYVELIN QAARLNGYVD AGDSWRSMYE TPSLEQDLER LFQELQPLYL

    NLHAYVRRAL HRHYGAQHIN LEGPIPAHLL GNMWAQTWSN IYDLVVPFPS

    APSMDTTEAM LKQGWTPRRM FKEADDFFTS LGLLPVPPEF WNKSMLEKPT

    DGREVVCHAS AWDFYNGKDF RIKQCTTVNL EDLVVAHHEM GHIQYFMQYK

    DLPVALREGA NPGFHEAIGD VLALSVSTPK HLHSLNLLSS EGGSDEHDIN

    FLMKMALDKI AFIPFSYLVD QWRWRVFDGS ITKENYNQEW WSLRLKYQGL

    CPPVPRTQGD FDPGAKFHIP SSVPYIRYFV SFIIQFQFHE ALCQAAGHTG

    PLHKCDIYQS KEAGQRLATA MKLGFSRPWP EAMQLITGQP NMSASAMLSY

    FKPLLDWLRT ENELHGEKLG WPQYNWTPNS ARSEGPLPDS GRVSFLGLDL

    DAQQARVGQW LLLFLGIALL VATLGLSQRL FSIRHRSLHR HSHGPQFGSE

    VELRHS

  • UCSC uc002jau.3; human. [P12821-1];


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