Human ANGPT4 ELISA Kit

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  • Alternative name

    Human ANG-4 ELISA Kit;Human Angiopoietin-3 ELISA Kit;Human ANG-3 ELISA Kit;Human ANG3 ELISA Kit;Human ANG4 ELISA Kit;Human angiopoietin 4 ELISA Kit;Human angiopoietin-4 ELISA Kit;

  • Catalog
    E002054
  • species
    Human
  • GeneANGPT4
  • Standard CurveHuman ANGPT4 ELISA Kit
  • Other Species Mouse Angpt4 ELISA KitBovine ANGPT4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiopoietin 4 (ANGPT4). No significant cross-reactivity or interference between Angiopoietin 4 (ANGPT4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 8.22pg/mL.
  • Intended UseHuman ANGPT4 ELISA Kit allows for the in vitro quantitative determination of ANGPT4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin 4 (ANGPT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin 4 (ANGPT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin 4 (ANGPT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin 4 (ANGPT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Angiopoietin-4 Protein information
  • Uniprot ID ANGP4_HUMAN
  • Uniprot AC Q9Y264; B4E3J9; Q5TFF4; Q9H4Z4;
  • UniGene Hs.278973; Hs.565714;
  • GeneID 51378
  • KEGG hsa:51378;
  • Human Angiopoietin-4 Protein SEQUENCE
  • SEQUENCE 503 AA; 56849 MW; 79CE52F66B532340 CRC64;

    MLSQLAMLQG SLLLVVATMS VAQQTRQEAD RGCETLVVQH GHCSYTFLLP

    KSEPCPPGPE VSRDSNTLQR ESLANPLHLG KLPTQQVKQL EQALQNNTQW

    LKKLERAIKT ILRSKLEQVQ QQMAQNQTAP MLELGTSLLN QTTAQIRKLT

    DMEAQLLNQT SRMDAQMPET FLSTNKLENQ LLLQRQKLQQ LQGQNSALEK

    RLQALETKQQ EELASILSKK AKLLNTLSRQ SAALTNIERG LRGVRHNSSL

    LQDQQHSLRQ LLVLLRHLVQ ERANASAPAF IMAGEQVFQD CAEIQRSGAS

    ASGVYTIQVS NATKPRKVFC DLQSSGGRWT LIQRRENGTV NFQRNWKDYK

    QGFGDPAGEH WLGNEVVHQL TRRAAYSLRV ELQDWEGHEA YAQYEHFHLG

    SENQLYRLSV VGYSGSAGRQ SSLVLQNTSF STLDSDNDHC LCKCAQVMSG

    GWWFDACGLS NLNGVYYHAP DNKYKMDGIR WHYFKGPSYS LRASRMMIRP

    LDI

  • UCSC uc002wei.4; human. [Q9Y264-1];


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