Human IL12RB2 ELISA Kit

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  • Alternative name

    Human IL-12 receptor subunit beta-2 ELISA Kit;Human IL-12R subunit beta-2 ELISA Kit;Human IL-12R-beta-2 ELISA Kit;Human IL-12RB2 ELISA Kit;Human interleukin 12 receptor subunit beta 2 ELISA Kit;Human interleukin-12 receptor subunit beta-2 ELISA Kit;Human interleukin 12 receptor, beta 2 ELISA Kit;Human interleukin-12 receptor beta-2 chain ELISA Kit;

  • Catalog
    E020503
  • species
    Human
  • GeneIL12RB2
  • Standard CurveHuman IL12RB2 ELISA Kit
  • Other Species Mouse Il12rb2 ELISA KitBovine IL12RB2 ELISA KitPorcine IL12RB2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 12 Receptor Beta 2 (IL12Rb2). No significant cross-reactivity or interference between Interleukin 12 Receptor Beta 2 (IL12Rb2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman IL12RB2 ELISA Kit allows for the in vitro quantitative determination of IL12RB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 Receptor Beta 2 (IL12Rb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 Receptor Beta 2 (IL12Rb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 Receptor Beta 2 (IL12Rb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12 Receptor Beta 2 (IL12Rb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Interleukin-12 receptor subunit beta-2 Protein information
  • Uniprot ID I12R2_HUMAN
  • Uniprot AC Q99665; B1AN98; B7ZKL9; F5H7L6; Q2M3V3;
  • UniGene Hs.479347;
  • GeneID 3595
  • KEGG hsa:3595;
  • Human Interleukin-12 receptor subunit beta-2 Protein SEQUENCE
  • SEQUENCE 862 AA; 97135 MW; 67C0E0D946B8DD58 CRC64;

    MAHTFRGCSL AFMFIITWLL IKAKIDACKR GDVTVKPSHV ILLGSTVNIT

    CSLKPRQGCF HYSRRNKLIL YKFDRRINFH HGHSLNSQVT GLPLGTTLFV

    CKLACINSDE IQICGAEIFV GVAPEQPQNL SCIQKGEQGT VACTWERGRD

    THLYTEYTLQ LSGPKNLTWQ KQCKDIYCDY LDFGINLTPE SPESNFTAKV

    TAVNSLGSSS SLPSTFTFLD IVRPLPPWDI RIKFQKASVS RCTLYWRDEG

    LVLLNRLRYR PSNSRLWNMV NVTKAKGRHD LLDLKPFTEY EFQISSKLHL

    YKGSWSDWSE SLRAQTPEEE PTGMLDVWYM KRHIDYSRQQ ISLFWKNLSV

    SEARGKILHY QVTLQELTGG KAMTQNITGH TSWTTVIPRT GNWAVAVSAA

    NSKGSSLPTR INIMNLCEAG LLAPRQVSAN SEGMDNILVT WQPPRKDPSA

    VQEYVVEWRE LHPGGDTQVP LNWLRSRPYN VSALISENIK SYICYEIRVY

    ALSGDQGGCS SILGNSKHKA PLSGPHINAI TEEKGSILIS WNSIPVQEQM

    GCLLHYRIYW KERDSNSQPQ LCEIPYRVSQ NSHPINSLQP RVTYVLWMTA

    LTAAGESSHG NEREFCLQGK ANWMAFVAPS ICIAIIMVGI FSTHYFQQKV

    FVLLAALRPQ WCSREIPDPA NSTCAKKYPI AEEKTQLPLD RLLIDWPTPE

    DPEPLVISEV LHQVTPVFRH PPCSNWPQRE KGIQGHQASE KDMMHSASSP

    PPPRALQAES RQLVDLYKVL ESRGSDPKPE NPACPWTVLP AGDLPTHDGY

    LPSNIDDLPS HEAPLADSLE ELEPQHISLS VFPSSSLHPL TFSCGDKLTL

    DQLKMRCDSL ML

  • UCSC uc001ddu.3; human. [Q99665-1];



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