Human IL12 ELISA Kit

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  • Alternative name

    Interleukin-12 subunit alpha ELISA KIT; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA KIT; CLMF p35 ELISA KIT; IL-12 subunit p35 ELISA KIT; NK cell stimulatory factor chain 1 ELISA KIT; NKSF1IL12A ELISA KIT; NKSF1 ELISA KIT; IL-12A ELISA KIT; CLMF p35 ELISA KIT; NKSF1 ELISA KIT

  • Catalog
  • species
  • GeneIL12
  • Standard CurveHuman IL12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 12 (IL12). No significant cross-reactivity or interference between Interleukin 12 (IL12) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.28pg/mL..
  • Intended UseHuman IL12 ELISA Kit allows for the in vitro quantitative determination of IL12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 (IL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 (IL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 (IL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12 (IL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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